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HRA-19a1.1 Alpha2Alpha1 B Cell Line

Info

Antigen/Gene or Protein Targets Chimeric alpha2 integrin (extracellular and transmembrane domain of alpha2 integrin and cytoplasmic domain of alpha1 integrin)
Host Human
Disease Keywords Human colorectal adenocarcinoma
Relevance The HRA-19a1.1 Alpha2Alpha1 B cell line is a genetically modified clone of HRA-19a1.1 cells expressing a chimeric alpha2 integrin with the extracellular and transmembrane domain of alpha2 integrin and the cytoplasmic domain of alpha1 integrin. Integrins are transmembrane receptors that are the bridges for cell-cell and cell-extracellular matrix (ECM) interactions.
HRA-19 has stem cell properties and undergoes a multilineage differentiation in serum free medium.
Production Details Genetically modified HRA-19a1.1 cells expressing a chimeric alpha2 integrin with the extracellular and transmembrane domain of alpha2 integrin and the cytoplasmic domain of alpha1 integrin
Research Area Cancer, Cell Structure and Motility, Drug Discovery & Development, Stem Cell Biology
Growth/Phenotype Keywords Morphology: Epithelial Growth Mode: Adherent
Recommended Growing Conditions Subculture Routine: Split sub-confluent cultures (70-80%) 1:3 to 1:4 weekly using 0.0125% trypsin/EDTA; 7.5% CO2; 37°C. Suggested seeding density 2-4x10,000 cells/cm².Culture Medium: DMEM (High glucose) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + G418 200µg/ml
Notes DNA Profile: STR-PCR Data:
Amelogenin: X,Y
CSF1PO: 12
D13S317: 11,12
D16S539: 11
D5S818: 11
D7S820: 9,12
THO1: 8
TPOX: 11
vWA: 16
Genetically Modified Organism Class 1 (GMO1)
Cellosaurus ID CVCL_2510

References: 5 entries

Henderson et al. 1996. Differentiation. 60(4):259-68. PMID: 8765056.

Kirkland et al. 1986. Br J Cancer. 53(6):779-85. PMID: 3718830.

Kirkland et al. 1986. Differentiation. 33(2):148-55. PMID: 3569697.

Kirkland et al. 1988. Cancer. 61(7):1359-63. PMID: 2449944.

Kirkland et al. 2008. J Biol Chem. 283(41):27612-9. PMID: 18664572.


Add a reference

References: 5 entries

Henderson et al. 1996. Differentiation. 60(4):259-68. PMID: 8765056.

Kirkland et al. 1986. Br J Cancer. 53(6):779-85. PMID: 3718830.

Kirkland et al. 1986. Differentiation. 33(2):148-55. PMID: 3569697.

Kirkland et al. 1988. Cancer. 61(7):1359-63. PMID: 2449944.

Kirkland et al. 2008. J Biol Chem. 283(41):27612-9. PMID: 18664572.


Add a reference

References: 5 entries

Henderson et al. 1996. Differentiation. 60(4):259-68. PMID: 8765056.

Kirkland et al. 1986. Br J Cancer. 53(6):779-85. PMID: 3718830.

Kirkland et al. 1986. Differentiation. 33(2):148-55. PMID: 3569697.

Kirkland et al. 1988. Cancer. 61(7):1359-63. PMID: 2449944.

Kirkland et al. 2008. J Biol Chem. 283(41):27612-9. PMID: 18664572.


Add a reference


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