A:
A polyclonal antibody preparation consists of a mixture of different antibodies, also known as immunoglobulins, which bind to different parts of the same target.
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Q: Can I use Plasmax alone or is it good to add FBS?
A:
Plasmax does not contain any proteins, growth factors or lipid therefore FBS should be added at the concentration you consider most appropriate.
Read moreQ: Can Plasmax be supplemented with antibiotics?
A:
Yes
Read moreQ: KCGS FAQs
A:
For more information on KCGS or for commonly asked questions, please visit https://www.sgc-unc.org/faq
Q: What are the advantages/ disadvantages of including serum in media?
A:
As serum is derived from natural tissues, adding serum to your cell culture growth media, provides your cell culture with some physiological elements. However, there are also disadvantages, particularly as the exact composition of serum is undefined.
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Q: What is the difference between primary and secondary cell culture?
A:
A primary cell culture refers to cells that have been removed from a live donor/specimen and have been established in an in vitro environment. Secondary cell culture refers to cell lines that have been immortalized and can divide indefinitely.
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Q: How long does cell culture media last?
A:
In general, cell culture media lasts for 3-4 weeks once it has been opened and supplemented, though this will vary across cell culture media and the rate of usage
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Q: What is the role of trace elements in Plasmax?
A:
Plasmax is the only ready-to-use cell culture medium containing growth enhancing components at physiological levels as well as essential trace elements.
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Supporting material
Videos (1)Q: How often should you change your cell culture media?
A:
Depending on the cell type, media should be changed every 2-3 days, which normally coincides with what is called passaging.
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Q: Cell culture tips and tricks
A:
Discover a range of cell culture tips and tricks
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Q: How do I select the right cell culture media?
A:
Cell culture media have been targeted to specific cell culture types and have been tailored to provide a variety of different formulation options, so selecting the most appropriate cell type for your research is a crucial first step.
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