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Anti-Cdk1 [17 (A17)]

Invented by Julian Gannon at The Francis Crick Institute

Info

Catalogue Number 151037
Applications ELISA IHC IP WB
Antigen/Gene or Protein Targets Cyclin dependent kinase 1 (Cdk1, cdc2)
Synonyms Cyclin-Dependent Kinase 1; Cell Division Cycle 2, G1 To S And G2 To M; Cell Division Control Protein 2 Homolog; Cell Division Protein Kinase 1; P34 Protein Kinase; P34CDC2; CDC28A; CDC2; Cell Cycle Controller CDC2; CDKN1
Reactivity Chicken, Human, Mouse, Rat and Xenopus laevis
Relevance Cdk1 (Cdc2) is a serine/threonine kinase which forms complexes with cyclins A and B which then phosphorylate a variety of target substrates to enable cell cycle progression. Therefore, Cdk1 is a marker for proliferating tumour cells.
Host Mouse
Immunogen A carboxy-terminal fragment starting at methionine 85 of p34 cdc2 (Xenopus laevis).
Subclass IgG2a
Molecular Weight (kDa) 34
Myeloma Used Sp2/0-Ag14
Recommended Growing Conditions DMEM + 5% FCS.
Strain Balb/c
Notes Recommended for formulin fixed immunohistochemistry samples.

Tested Applications Dilution

Radioimmune Assays (RIA) Assay dependent

Immunocytochemistry (ICC) 5 µg/ml

Immunomicroscopy (IM) Assay-dependent

ELISA (ELISA) Assay dependent

Immunoprecipitation (IP) 2.5 µg/I

Western Blot (WB) 1µg/ml

Immunofluorescence (IF) Assay dependent

Immunohistochemistry (Paraffin, Frozen) (IHC (P, F)) 5 µg/ml
Research Area Cancer, Cell Cycle, Cell Type or Organelle Marker, Neurobiology

References: 12 entries

Zhou et al. 2015. Platelets. :1-11. PMID: 26325015.

Platelets promote cartilage repair and chondrocyte proliferation via ADP in a rodent model of osteoarthritis.

Europe PMC ID: 26325015

Coulonval et al. 2011. Mol Biol Cell. 22(21):3971-85. PMID: 21900495.

Coupling of T161 and T14 phosphorylations protects cyclin B-CDK1 from premature activation.

Europe PMC ID: 21900495

Gannon et al. 1998. Genes Cells. 3(1):17-27. PMID: 9581979.

A measure of the mitotic index: studies of the abundance and half-life of p34cdc2 in cultured cells and normal and neoplastic tissues.

Europe PMC ID: 9581979

Goodger et al. 1996. J Pathol. 178(4):422-8. PMID: 8691321.

The localization of p34cdc2 in the cells of normal, hyperplastic, and malignant epithelial and lymphoid tissues of the oral cavity.

Europe PMC ID: 8691321

Doussis-Anagnostopoulou et al. 1994. Histopathology. 24(4):335-40. PMID: 8045523.

Distribution of the cdc2 gene product in normal tissues: an immunocytochemical study using four new monoclonal antibodies.

Europe PMC ID: 8045523

Kobayashi et al. 1992. Mol Biol Cell. 3(11):1279-94. PMID: 1333843.

Identification of the domains in cyclin A required for binding to, and activation of, p34cdc2 and p32cdk2 protein kinase subunits.

Europe PMC ID: 1333843


Add a reference

References: 12 entries

Zhou et al. 2015. Platelets. :1-11. PMID: 26325015.

Platelets promote cartilage repair and chondrocyte proliferation via ADP in a rodent model of osteoarthritis.

Europe PMC ID: 26325015

Coulonval et al. 2011. Mol Biol Cell. 22(21):3971-85. PMID: 21900495.

Coupling of T161 and T14 phosphorylations protects cyclin B-CDK1 from premature activation.

Europe PMC ID: 21900495

Gannon et al. 1998. Genes Cells. 3(1):17-27. PMID: 9581979.

A measure of the mitotic index: studies of the abundance and half-life of p34cdc2 in cultured cells and normal and neoplastic tissues.

Europe PMC ID: 9581979

Goodger et al. 1996. J Pathol. 178(4):422-8. PMID: 8691321.

The localization of p34cdc2 in the cells of normal, hyperplastic, and malignant epithelial and lymphoid tissues of the oral cavity.

Europe PMC ID: 8691321

Doussis-Anagnostopoulou et al. 1994. Histopathology. 24(4):335-40. PMID: 8045523.

Distribution of the cdc2 gene product in normal tissues: an immunocytochemical study using four new monoclonal antibodies.

Europe PMC ID: 8045523

Kobayashi et al. 1992. Mol Biol Cell. 3(11):1279-94. PMID: 1333843.

Identification of the domains in cyclin A required for binding to, and activation of, p34cdc2 and p32cdk2 protein kinase subunits.

Europe PMC ID: 1333843


Add a reference

References: 12 entries

Zhou et al. 2015. Platelets. :1-11. PMID: 26325015.

Platelets promote cartilage repair and chondrocyte proliferation via ADP in a rodent model of osteoarthritis.

Coulonval et al. 2011. Mol Biol Cell. 22(21):3971-85. PMID: 21900495.

Coupling of T161 and T14 phosphorylations protects cyclin B-CDK1 from premature activation.

Gannon et al. 1998. Genes Cells. 3(1):17-27. PMID: 9581979.

A measure of the mitotic index: studies of the abundance and half-life of p34cdc2 in cultured cells and normal and neoplastic tissues.

Goodger et al. 1996. J Pathol. 178(4):422-8. PMID: 8691321.

The localization of p34cdc2 in the cells of normal, hyperplastic, and malignant epithelial and lymphoid tissues of the oral cavity.

Doussis-Anagnostopoulou et al. 1994. Histopathology. 24(4):335-40. PMID: 8045523.

Distribution of the cdc2 gene product in normal tissues: an immunocytochemical study using four new monoclonal antibodies.

Kobayashi et al. 1992. Mol Biol Cell. 3(11):1279-94. PMID: 1333843.

Identification of the domains in cyclin A required for binding to, and activation of, p34cdc2 and p32cdk2 protein kinase subunits.


Add a reference


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