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Monoclonal Antibodies

Anti-HSVUL42 [2H4]

Invented at Cancer Research UK London Research Institute: Clare Hall Laboratories
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Adapted from Chen Y, Livingston CM, Carrington-Lawrence SD, Bai P, Weller SK. A mutation in the human herpes simplex virus type 1 UL52 zinc finger motif results in defective primase activity but can recruit viral polymerase and support viral replication efficiently. J Virol. 2007 Aug;81(16):8742-51. doi: 10.1128/JVI.00174-07. Epub 2007 Jun 6. PMID: 17553899; PMCID: PMC1951384. UL30, but not UL42, is extracted by detergent from replication compartments. Vero cells were plated on glass coverslips and infected the following day with 10 PFU/cell KOS. At 6 h p.i., cells were incubated for 2 min in either cold PBS (A and B) or cold 0.5% Triton X-100 cytoskeletal extraction buffer (C and D), followed by fixation and permeabilization for IF analysis as described in Materials and Methods. Cells were probed with monoclonal mouse anti-UL30 antibody 1051b (A and C) or monoclonal mouse anti-UL42 (B and D). Each image shows a representative KOS-infected cell, with images taken at the same settings for each primary antibody.
Adapted from Chen Y, Livingston CM, Carrington-Lawrence SD, Bai P, Weller SK. A mutation in the human herpes simplex virus type 1 UL52 zinc finger motif results in defective primase activity but can recruit viral polymerase and support viral replication efficiently. J Virol. 2007 Aug;81(16):8742-51. doi: 10.1128/JVI.00174-07. Epub 2007 Jun 6. PMID: 17553899; PMCID: PMC1951384.The viral polymerase UL30 was detergent-extracted from HSV-1-infected cells. Vero cells were either mock infected or infected with HSV WT strain KOS for 6 h. Cells were treated with either PBS or 0.5% Triton X-100 extraction buffer, followed by Western blotting using antisera raised against UL30 (top), UL42 (middle), or γ-tubulin (bottom) as described in Materials and Methods.
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Catalogue Number 151043
Applications ELISA IHC IF IP WB ICC
Antigen/Gene or Protein Targets Herpes Simplex Virus protein UL42 (HSV-UL42)
Reactivity Virus
Relevance UL42 is the DNA polymerase accessory protein of herpes simplex virus type 1. 2H4 may be used to detect UL42 bound to HSV polymerase. Recognises amino acids 360-366 in UL42.
Host Mouse
Immunogen Baculovirus-expressed HSV DNA polymerase (POL) and POL/UL42 complex.
Subclass IgG1
Myeloma Used Sp2/0-Ag14
Recommended Growing Conditions DMEM + 5% FCS
Strain Balb/c
Notes Reactivity: Herpes simplex virus
Research Area Virology

References

There are 6 reference entries for this reagent.

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References: 6 entries

Lv et al. 2018. Antiviral Res. 159:55-62. PMID: 30266338.

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Döhner et al. 2018. PLoS Pathog. 14(1):e1006823. PMID: 29304174.

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Dembowski et al. 2017. PLoS Pathog. 13(1):e1006166. PMID: 28095497.

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Chen et al. 2007. J Virol. 81(16):8742-51. PMID: 17553899.

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Chen et al. 2007. J Virol. 81(16):8742-51. PMID: 17553899.

IF WB ICC

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A mutation in the human herpes simplex virus type 1 UL52 zinc finger motif results in defective primase activity but can recruit viral polymerase and support viral replication efficiently.

Europe PMC ID: 17553899

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References: 6 entries

Lv et al. 2018. Antiviral Res. 159:55-62. PMID: 30266338.

View  

Döhner et al. 2018. PLoS Pathog. 14(1):e1006823. PMID: 29304174.

View  

Dembowski et al. 2017. PLoS Pathog. 13(1):e1006166. PMID: 28095497.

View  

Chen et al. 2007. J Virol. 81(16):8742-51. PMID: 17553899.

View  

Chen et al. 2007. J Virol. 81(16):8742-51. PMID: 17553899.

IF WB ICC

View  

A mutation in the human herpes simplex virus type 1 UL52 zinc finger motif results in defective primase activity but can recruit viral polymerase and support viral replication efficiently.

View  

Add a reference

Inventor Information