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Anti-CD11c (Integrin aX) [3.9]
RECENTLY UPDATED

Invented by Prof Nancy Hogg at Cancer Research UK London Research Institute: Lincoln's Inn Fields

Info

Catalogue Number 151044
Applications FACS IHC IF IP Fn
Antigen/Gene or Protein Targets Integrin alpha X (CD11c; gp150/95 a subunit)
Synonyms CD11 antigen-like family member C, Leu M5, Leukocyte adhesion glycoprotein p150,95 alpha chain, Leukocyte adhesion receptor p150,95, CD antigen: CD11c
Reactivity Human
Relevance
RECENTLY UPDATED
Monoclonal antibody directed against CD11c antigen detects presence of macrophages, mononuclear phagocytes, hairy cell leukaemia cells and various activated T cells and neutrophils.

Background and Research Application
Anti-CD11c (integrin aX) was created to understand the expression of the integrin aX molecule and to look at the heterogeneity of tissue macrophages.
Integrins are heterodimeric cell surface receptors composed of alpha and beta subunits, which mediate cell-cell and cell-extracellular matrix attachments. Changes in integrin expression have been shown to be important for the growth and early metastatic capacity of melanoma cells and aberrant integrin expression has been found in many epithelial tumours. Integrin alpha X is found on hairy cell leukaemia’s, malignant histiocytes, on tissue macrophages, monocytes, activated neutrophils and some T-cell clones. It is a member of the LFA-1 CR3 family of human leukocyte differentiation antigens. This antibody binds to CD11c glycoprotein which helps mediate cellular adhesion and cell-matrix adhesion such as fibronectin.
Host Mouse
Immunogen Rheumatoid synovial fluid cells and fibronectin purified human monocytes
Immunogen UniProt ID
RECENTLY UPDATED
P20702
Positive Control Peripheral blood monocytes (PBMCs) (FACS), Uterine tissue sections (IHC)
Subclass IgG1
Molecular Weight (kDa) 150
Myeloma Used Sp2/0-Ag14
Recommended Growing Conditions DMEM + 5% FCS
Strain Balb/c
Notes
RECENTLY UPDATED
Production Details
Purified using multi-step affinity chromatography with protein A.

Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.

Points of Interest
The epitope which anti-CD11c 3(.9) detects is present on a non-covalently linked dimer of 150000 and 95000 and is directed against the alpha chain of the integrin aX molecule. This antibody reacts variably with neutrophils, more strongly with monocytes and most strong on macrophages. It does not react with Langerhans cells, dendritic reticulum cells or osteoclasts. Anti-CD11c antibody can be used as a marker of mononuclear phagocytes, and for CD11c expression on monocytic cells.

Shown to inhibit adhesion in Jawhara et al., 2012.

For FACS: 1ug per 1x10^6 cells

Concentration
1mg/ml as standard
Research Area Adhesion, Cell Signaling & Signal Transduction, Immunology, Stem Cell Biology

References

There are 28 reference entries for this reagent.

View All References

References: 28 entries
RECENTLY UPDATED

Langer et al. 2019. Elife. 8:. PMID: 30717826.

Dos Reis et al. 2019. Front Immunol. 10:1291. PMID: 31244842.

Hunegnaw et al. 2019. Front Immunol. 10:1537. PMID: 31333668.

Muir et al. 2018. J Leukoc Biol. 103(4):615-628. PMID: 29493813.

Silveira et al. 2017. J Immunol. 199(3):1003-1011. PMID: 28667164.

Locci et al. 2016. Nat Immunol. :. PMID: 27376469.

IF

Activin A programs the differentiation of human TFH cells.

Europe PMC ID: 27376469

Göbel et al. 2016. Nat Commun. 7:11626. PMID: 27188843.

FACS IHC

Blood coagulation factor XII drives adaptive immunity during neuroinflammation via CD87-mediated modulation of dendritic cells.

Europe PMC ID: 27188843

Expression of death receptor 3 on peripheral blood mononuclear cells differes in adult IBD patients and children with newly diagnosed IBD.

Europe PMC ID: 27001939

Dendritic Cell-Secreted Cytotoxic T-Lymphocyte-Associated Protein-4 Regulates the T-cell Response by Downmodulating Bystander Surface B7.

Europe PMC ID: 26979751

Slebioda et al. 2016. Cytometry B Clin Cytom. :. PMID: 27001939.

FACS

Halpert et al. 2016. Stem Cells Dev. :. PMID: 26979751.

Alginate microsphere compositions dictate different mechanisms of complement activation with consequences for cytokine release and leukocyte activation.

Europe PMC ID: 26993426

Ørning et al. 2016. J Control Release. :. PMID: 26993426.

Swindle et al. 2015. Immunology. 146(3):470-85. PMID: 26288256.

Increased proportions of dendritic cells and recovery of IFNγ responses in HIV/HCV co-infected patients receiving ART.

Europe PMC ID: 26455474

Saraswati et al. 2015. Hum Immunol. :. PMID: 26455474.

Interferon-γ enhances both the anti-bacterial and the pro-inflammatory response of human mast cells to Staphylococcus aureus.

Europe PMC ID: 26288256

Petvises et al. 2015. Cell Mol Immunol. :. PMID: 25891217.

Delineation of a novel dendritic-like subset in human spleen.

Europe PMC ID: 25891217

Nausch et al. 2015. PLoS Negl Trop Dis. 9(3):e0003627. PMID: 25799270.

Group 2 innate lymphoid cell proportions are diminished in young helminth infected children and restored by curative anti-helminthic treatment.

Europe PMC ID: 25799270

Jawhara et al. 2012. J Immunol. 189(5):2468-77. PMID: 22844116.

Integrin αXβ₂ is a leukocyte receptor for Candida albicans and is essential for protection against fungal infections.

Europe PMC ID: 22844116

McMichael AJ et al. (Ed) 1997. Leucocyte Typing III. ISBN 13 9780192615527

Hogg et al. 1986. Eur J Immunol. 16(3):240-8. PMID: 3456894.

The p150,95 molecule is a marker of human mononuclear phagocytes: comparison with expression of class II molecules.

Europe PMC ID: 3456894


Add a reference

References: 28 entries
RECENTLY UPDATED

Langer et al. 2019. Elife. 8:. PMID: 30717826.

Dos Reis et al. 2019. Front Immunol. 10:1291. PMID: 31244842.

Hunegnaw et al. 2019. Front Immunol. 10:1537. PMID: 31333668.

Muir et al. 2018. J Leukoc Biol. 103(4):615-628. PMID: 29493813.

Silveira et al. 2017. J Immunol. 199(3):1003-1011. PMID: 28667164.

Locci et al. 2016. Nat Immunol. :. PMID: 27376469.

IF

Activin A programs the differentiation of human TFH cells.

Göbel et al. 2016. Nat Commun. 7:11626. PMID: 27188843.

FACS IHC

Blood coagulation factor XII drives adaptive immunity during neuroinflammation via CD87-mediated modulation of dendritic cells.

Expression of death receptor 3 on peripheral blood mononuclear cells differes in adult IBD patients and children with newly diagnosed IBD.

Dendritic Cell-Secreted Cytotoxic T-Lymphocyte-Associated Protein-4 Regulates the T-cell Response by Downmodulating Bystander Surface B7.

Slebioda et al. 2016. Cytometry B Clin Cytom. :. PMID: 27001939.

FACS

Halpert et al. 2016. Stem Cells Dev. :. PMID: 26979751.

Alginate microsphere compositions dictate different mechanisms of complement activation with consequences for cytokine release and leukocyte activation.

Ørning et al. 2016. J Control Release. :. PMID: 26993426.

Swindle et al. 2015. Immunology. 146(3):470-85. PMID: 26288256.

Increased proportions of dendritic cells and recovery of IFNγ responses in HIV/HCV co-infected patients receiving ART.

Saraswati et al. 2015. Hum Immunol. :. PMID: 26455474.

Interferon-γ enhances both the anti-bacterial and the pro-inflammatory response of human mast cells to Staphylococcus aureus.

Petvises et al. 2015. Cell Mol Immunol. :. PMID: 25891217.

Delineation of a novel dendritic-like subset in human spleen.

Nausch et al. 2015. PLoS Negl Trop Dis. 9(3):e0003627. PMID: 25799270.

Group 2 innate lymphoid cell proportions are diminished in young helminth infected children and restored by curative anti-helminthic treatment.

Jawhara et al. 2012. J Immunol. 189(5):2468-77. PMID: 22844116.

Integrin αXβ₂ is a leukocyte receptor for Candida albicans and is essential for protection against fungal infections.

McMichael AJ et al. (Ed) 1997. Leucocyte Typing III. ISBN 13 9780192615527

Hogg et al. 1986. Eur J Immunol. 16(3):240-8. PMID: 3456894.

The p150,95 molecule is a marker of human mononuclear phagocytes: comparison with expression of class II molecules.


Add a reference