Anti-Arabinogalactan-protein [LM2] monoclonal antibody
Invented by Prof Paul Knox from PlantProbes
Invented at University of Leeds
- Datasheet
- References (1)
- Inventor Info
Info
| Catalogue Number | 157932 |
| Antigen/Gene or Protein Targets | Arabinogalactan-protein |
| Synonyms | Rice (Oryza Sativa) arabinogalactan-proteins |
| Relevance |
Arabinogalactan-proteins (AGPs) are a class of abundant proteoglycans found at the plant cell surface and are predominantly associated with the plasma membrane and extracellular secretions. Although the functions of AGPs are unknown it has been speculated that they may have roles as lubricants, in nutrition or in some aspect of plant cell recognition (Clarke et al, 1979; Fincher et al, 1983; Chasan, 1994). The addition of isolated AGPs to carrot cell cultures has been reported to influence development (Kreuger and van Hoist, 1993), and AGPs have been implicated in the control of plant cell proliferation (Basile and Basile, 1993; Serpe and Nothnagel, 1994). The generation and use of monoclonal antibodies recognizing arabinogalactan-proteins has demonstrated the extensive regulation of AGP carbohydrate epitopes in relation to early stages of cell development in roots, shoots and embryos further implicating this class of plant cell surface molecule in aspects of plant cell development (Knox et al, 1989; Pennell and Roberts, 1990; Stacey et al, 1990; Knox et al, 1991; Pennell et al, 1991; Schindler et al, 1995) |
| Host | Rat |
| Positive Control | IgM |
| Notes |
Generated to rice arabinogalactan-proteins (AGPs). Recognises a carbohydrate epitope containing b-linked glucuronic acid. It can recognise AGPs in several species. In competitive inhibition ELISAs antibody binding to gum arabic was inhibited (50%) by 70 µmg/ml 1-O-methyl-b-D-GlcA. The binding of the antibody to AGPs can be fully inhibited by 10 mM 1-O-methyl-b-D-GlcA. |
| Research Area | Plant Science |
