Cancer Research Technology
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Catalogue Number 151161
Applications ELISA FACS IHC
Antigen/Gene or Protein Targets Mucin1 (MUC1)
Synonyms ADMCKD, ADMCKD1, Breast carcinoma associated antigen DF3, Breast carcinoma-associated antigen DF3, CA 15-3, CA15 3, CA15 3 antigen, CA15.3, Cancer antigen 15-3, Carcinoma associated mucin, Carcinoma-associated mucin, CD 227, CD227
Reactivity Human
Relevance MUC1 is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoitic cell lineages. In the vast majority of human carcinomas, this protein is upregulated, poorly glycosylated and appears on the cell surface in a non-polarised fashion. SM3 antibody recognises this under-glycosylated form of MUC1 and is therefore tunour specific and may be relevant for breast cancer therapy. SM3 also detects MUC1 within colon and ovarian cancer and most adenocarcinomas and may be used for immunohistochemistry (reactive with methacarn fixed tissue), and immunotargeting.
Host Mouse
Immunogen Hydrogen fluoride deglycosylated milk mucin.
Subclass IgG2a kappa
Positive Control MCF7 cells (FACS) or human breast carcinoma (IHC)
Myeloma Used P3X63Ag8.653
Recommended Growing Conditions RPMI 1640 + 10% FCS
Strain Balb/c
Notes The product of the MUC1 gene is the polymorphic epithelial mucin. SM3 reacts with a core protein epitope defined as PDTRP.
Research Area Cancer, Cell Type or Organelle Marker, Cell Signaling & Signal Transduction

References: 12 entries

Geng et al. 2013. Cell Mol Bioeng. 6(2):148-159. PMID: 23805168.

Targeting Underglycosylated MUC1 for the Selective Capture of Highly Metastatic Breast Cancer Cells Under Flow.

Europe PMC ID: 23805168

Sørensen et al. 2006. Glycobiology. 16(2):96-107. PMID: 16207894.

Chemoenzymatically synthesized multimeric Tn/STn MUC1 glycopeptides elicit cancer-specific anti-MUC1 antibody responses and override tolerance.

Europe PMC ID: 16207894

Brockhausen et al. 1995. Eur J Biochem. 233(2):607-17. PMID: 7588808.

Mechanisms underlying aberrant glycosylation of MUC1 mucin in breast cancer cells.

Europe PMC ID: 7588808

Burchell et al. 1993. Epithelial Cell Biol. 2(4):155-62. PMID: 7505698.

FACS

Effect of modification of carbohydrate side chains on the reactivity of antibodies with core-protein epitopes of the MUC1 gene product.

Europe PMC ID: 7505698

Burchell et al. 1989. Int J Cancer. 44(4):691-6. PMID: 2477336.

A short sequence, within the amino acid tandem repeat of a cancer-associated mucin, contains immunodominant epitopes.

Europe PMC ID: 2477336

Burchell et al. 1987. Cancer Res. 47(20):5476-82. PMID: 2443241.

Development and characterization of breast cancer reactive monoclonal antibodies directed to the core protein of the human milk mucin.

Europe PMC ID: 2443241


Add a reference

References: 12 entries

Geng et al. 2013. Cell Mol Bioeng. 6(2):148-159. PMID: 23805168.

Targeting Underglycosylated MUC1 for the Selective Capture of Highly Metastatic Breast Cancer Cells Under Flow.

Europe PMC ID: 23805168

Sørensen et al. 2006. Glycobiology. 16(2):96-107. PMID: 16207894.

Chemoenzymatically synthesized multimeric Tn/STn MUC1 glycopeptides elicit cancer-specific anti-MUC1 antibody responses and override tolerance.

Europe PMC ID: 16207894

Brockhausen et al. 1995. Eur J Biochem. 233(2):607-17. PMID: 7588808.

Mechanisms underlying aberrant glycosylation of MUC1 mucin in breast cancer cells.

Europe PMC ID: 7588808

Burchell et al. 1993. Epithelial Cell Biol. 2(4):155-62. PMID: 7505698.

FACS

Effect of modification of carbohydrate side chains on the reactivity of antibodies with core-protein epitopes of the MUC1 gene product.

Europe PMC ID: 7505698

Burchell et al. 1989. Int J Cancer. 44(4):691-6. PMID: 2477336.

A short sequence, within the amino acid tandem repeat of a cancer-associated mucin, contains immunodominant epitopes.

Europe PMC ID: 2477336

Burchell et al. 1987. Cancer Res. 47(20):5476-82. PMID: 2443241.

Development and characterization of breast cancer reactive monoclonal antibodies directed to the core protein of the human milk mucin.

Europe PMC ID: 2443241


Add a reference

References: 12 entries

Geng et al. 2013. Cell Mol Bioeng. 6(2):148-159. PMID: 23805168.

Targeting Underglycosylated MUC1 for the Selective Capture of Highly Metastatic Breast Cancer Cells Under Flow.

Sørensen et al. 2006. Glycobiology. 16(2):96-107. PMID: 16207894.

Chemoenzymatically synthesized multimeric Tn/STn MUC1 glycopeptides elicit cancer-specific anti-MUC1 antibody responses and override tolerance.

Brockhausen et al. 1995. Eur J Biochem. 233(2):607-17. PMID: 7588808.

Mechanisms underlying aberrant glycosylation of MUC1 mucin in breast cancer cells.

Burchell et al. 1993. Epithelial Cell Biol. 2(4):155-62. PMID: 7505698.

FACS

Effect of modification of carbohydrate side chains on the reactivity of antibodies with core-protein epitopes of the MUC1 gene product.

Burchell et al. 1989. Int J Cancer. 44(4):691-6. PMID: 2477336.

A short sequence, within the amino acid tandem repeat of a cancer-associated mucin, contains immunodominant epitopes.

Burchell et al. 1987. Cancer Res. 47(20):5476-82. PMID: 2443241.

Development and characterization of breast cancer reactive monoclonal antibodies directed to the core protein of the human milk mucin.


Add a reference


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