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Monoclonal Antibodies

Anti-Involucrin [SY8]

Invented by Prof Fiona Watt from King's College London
Invented at Cancer Research UK London Research Institute: Lincoln's Inn Fields
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Adapted from Howie et al. 2011. PLoS Pathog. 7(8):e1002211. PMID: 21901101 Figure. p300 degradation alters K1, K10 and IVL expression. (A) Representative immunoblot showing levels of K1, K10 and IVL protein in LXSN, 38E6, 8E6 and Δ8E6-expressing HFKs during 72 hr calcium-induced differentiation. Actin levels are shown as a loading control. (B) K1, K10 and IVL mRNA levels levels in LXSN 38E6, 8E6 and Δ8E6-expressing HFKs during 72 hr calcium-induced differentiation. Relative levels of each mRNA were calculated using the ΔΔCT method with GAPDH to normalize mRNA levels within each sample. Values shown are fold-change in each sample compared to the LXSN (0 hr) vector control. (C) ChIP analysis using anti-p300 antibodies to pull down chromatin from LXSN control, 8E6 and 38E6-expressing HFKs, with quantitation by real-time PCR. Values represent the enrichment of p300 at the IVL promoter shown relative to the IgG and RPL30 negative controls. Error bars represent standard deviation (n = 3, p<.01 by 2-tailed student's T-test).
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Catalogue Number 151164
Applications IHC IF IP WB
Antigen/Gene or Protein Targets Involucrin
Reactivity Dog, Human, Pig and Primate
Relevance Involucrin is a protein precursor of the epidermal cornified envelope and a differentiation marker of human keratinocytes. SY8 is useful for studies of changes in involucrin expression in pathological studies.
Host Mouse
Immunogen Purified involucrin from human keratinocytes.
Subclass IgG1
Myeloma Used P3X63Ag8
Recommended Growing Conditions DMEM + 5% FCS
Strain Balb/c
Notes There is weak reactivity with Canine
Research Area Cancer, Cell Signaling & Signal Transduction

References

There are 4 reference entries for this reagent.

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References: 4 entries

Howie et al. 2011. PLoS Pathog. 7(8):e1002211. PMID: 21901101.

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Beta-HPV 5 and 8 E6 promote p300 degradation by blocking AKT/p300 association.

Europe PMC ID: 21901101

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Hudson et al. 1992. Hybridoma. 11(3):367-79. PMID: 1500072.

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Characterisation of eight monoclonal antibodies to involucrin.

Europe PMC ID: 1500072

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References: 4 entries

Howie et al. 2011. PLoS Pathog. 7(8):e1002211. PMID: 21901101.

View  

Beta-HPV 5 and 8 E6 promote p300 degradation by blocking AKT/p300 association.

View  

Hudson et al. 1992. Hybridoma. 11(3):367-79. PMID: 1500072.

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Characterisation of eight monoclonal antibodies to involucrin.

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Inventor Information