Cancer Research Technology
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Catalogue Number 152723
Backbone Size (bp) 5169
Bacterial Resistance Ampicillin
Vector Type pBabe-puro
Antigen/Gene or Protein Targets Rock2, RockII
Relevance Rock2:ER Plasmid contains the catalytic domain of human Rock-II fused at the carboxy-terminal end to a mutated ligand binding domain of the estrogen receptor (ER) and also to enhanced green fluorescence protein at the amino terminus. When expressed the Rock2:ER fusion protein is inactive because of the binding of a heat shock protein (HSP90) to the ER ligand binding domain. Addition of the estrogen homolog, 4-hydroxytamoxifen (4-HT), results in displacement of HSP90 and activation of the kinase.
Research Area Cancer, Cell Signaling & Signal Transduction, Cell Structure and Motility

References

There are 4 reference entries for this reagent.

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References: 4 entries

Croft et al. 2004. Cancer Res. 64(24):8994-9001. PMID: 15604264.

Conditional ROCK activation in vivo induces tumor cell dissemination and angiogenesis.

Europe PMC ID: 15604264

McMullan et al. 2003. Curr Biol. 13(24):2185-9. PMID: 14680635.

Keratinocyte differentiation is regulated by the Rho and ROCK signaling pathway.

Europe PMC ID: 14680635


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References: 4 entries

Croft et al. 2004. Cancer Res. 64(24):8994-9001. PMID: 15604264.

Conditional ROCK activation in vivo induces tumor cell dissemination and angiogenesis.

McMullan et al. 2003. Curr Biol. 13(24):2185-9. PMID: 14680635.

Keratinocyte differentiation is regulated by the Rho and ROCK signaling pathway.


Add a reference