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|Applications||ELISA IF WB|
|Antigen/Gene or Protein Targets||GSK-3 (SGG)|
The Monoclonal Anti-GSK-3 / Shaggy protein kinase family, Clone 7G-1F2 reacts with both the phosphorylated peptide and the non phosphorylated peptide and with the corresponding GSK-3/Shaggy proteins. The phosphorylated peptide used as an immunogen derives from the N-terminal of the shaggy polypeptide and is well conserved in comparable regions of the rat GSK-3- alpha and beta enzymes (position 8 is a T in GSK-3-beta, and position 5 is an A in GSK-3-alpha). The reactivity with this antibody is unchanged by insulin or EGF treatments of cells and can be used as a reference when detecting changes in phosphorylation on ser9 using
clone 7G-2G5. Glycogen synthase kinase 3 is a serine/threonine protein kinase that mediates the addition of phosphate molecules onto serine and threonine amino acid residues. Phosphorylation of a protein by GSK-3 usually inhibits the activity of its downstream target. GSK-3 is active in a number of central intracellular signaling pathways, including cellular proliferation, migration, glucose regulation, and apoptosis.
|Immunogen||Phosphorylated synthetic peptide coupled to ovalbumine : (C)SGRPRTSSFAE|
Available in 100ul unit as ascites.
Recommended dilutions : 1/500 - 1/5000
|Research Area||Apoptosis and Programmed Cell Death, Cancer, Cardiovascular, Cell Signaling & Signal Transduction, Metabolism|
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