Cancer Research Technology
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Catalogue Number 153871
Backbone Size (bp) 8018
Bacterial Resistance Ampicillin
Selectable Markers Puromycin
Vector Type Mammalian expression, CRISPR, shuttle
Antigen/Gene or Protein Targets Gt(ROSA)26Sor; ROSA26
Positive Control Contains iRFP720 for positive transfection/integration control
Relevance CRISPR donor vector targeting ROSA26 locus in mouse cells. Contains left and right ROSA26 homology arms with AsisI/MluI cloning site. Use for genomic integration of any gene of interest into ROSA26 locus.

Use in combination with pROSA-GUIDE containing validated gRNA for CAS9 targeting to ROSA26.


Species: Mus musculus (house mouse)
Tag: Myc, FLAG
Cloning method: Restriction Enzyme
5’ cloning site: AsiSI
3’ cloning site: MluI
5’ sequencing primer: CMV Forward
Research Area Gene Expression
Notes Cancer Research Technology Limited (trading research tools as Ximbio) has been granted a non-exclusive license to the CRISPR-Cas9 technology by ERS Genomics Ltd under the patent rights listed here.

This license from ERS Genomics Ltd allows Ximbio to develop and commercialise CRISPR-Cas9 modified cell lines and vectors for research use only. Ximbio can provide these CRISPR-Cas9 research tools to companies under a label-use only license.

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