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Anti-KAHRP [18.2]

Info

Applications ELISA IF WB
Antigen/Gene or Protein Targets Knob-associated histidine rich protein (KAHRP)
Reactivity Plasmodium
Relevance KAHRP (Plasmodium falciparum knob-associated histidine-rich protein) is a protein expressed in erythrocytes infected by Plasmodium falciparum. KAHRP is a major component of knobs, feature found on Plasmodium falciparum infected erythrocytes, and it contributes to their altered mechanical properties. The sequestration of infected erythrocytes in tissues represents an important factor in the course of fatalities in patients with malaria.
Host Mouse
Immunogen Schizont extracts of P.falciparum
Subclass IgG2b
Myeloma Used P3/NS1/1-Ag4.1
Strain Balb/c
Notes UK Biosafety level: 1
There are 35 SNPs in the KAHRP gene, in IF, mAb 18.2 reacts with isolates 3D7, MAD20, G1, PA17, and K1. Negative IFA results have been obtained with Dd2 and RO33 isolates.
Western blotting with schizont extracts from 3D7 parasites shows reactivity of mAb 18.2 with a ~ 90 kDa band. Warning: 3D7 parasites in long term culture may become 18.2 negative by IF – it is advised that proteins prepared for WB are from fresh (<20 generations) parasites.
WB conditions: Purify schizont proteinswith MACS column and resolve by SDS-PAGE (4-12% gel, Tris-glycine); blot onto nitrocellulose membrane. Incubate strips from the membrane with mAb 18.2 after blocking (5% non-fat milk in PBS) and detect with HRP-conjugated anti-mouse Ig using 3,3 diaminobenzidine substrate.
Research Area Parasitology

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