Anti-PLAP [HD 8B6]
Invented by Prof Walter Bodmer from University of Oxford
Invented at Cancer Research UK London Research Institute: Lincoln's Inn Fields
- Datasheet
- References (8)
- Inventor Info
Info
| Catalogue Number | 151107 |
| Applications | ELISA IHC IF RIA |
| Antigen/Gene or Protein Targets | Placental Alkaline Phosphatase (PLAP), Regan and Nagao isoenzymes. |
| Reactivity | Human |
| Relevance | Alkaline phosphatases are phosphodiesterases. The placental-specific isozyme of Alkaline Phosphatase (PLAP) is found in trophoblast cells of normal human mature placenta, seminomas of testis and ovarian carcinomas. The detection of alkaline phosphatase in serum is a marker for ovarian and testicular cancer. HD 8B6 has potential uses in the diagnosis of ovarian and testicular tumours. The antibody detects both Regan and Nagao isoenzymes. |
| Host | Mouse |
| Immunogen | Fresh Hep-2 cells followed by booster injections of fresh Hep-2 cells that have had their surface expression of PLAP boosted in culture. |
| Subclass | IgG2a |
| Myeloma Used | P3/NS1/1-Ag4.1 |
| Recommended Growing Conditions | DMEM + 5% FCS |
| Strain | Balb/c |
| Research Area | Cancer, Cell Type or Organelle Marker |
References: 8 entries
Sharp et al. 2010. PLoS One. 5(10):e13130. PMID: 20976190.
IF
Inhibition of cellular protein secretion by norwalk virus nonstructural protein p22 requires a mimic of an endoplasmic reticulum export signal.
Europe PMC ID: 20976190
Wojtowicz et al. 2007. Cell. 130(6):1134-45. PMID: 17889655.
ELISA
A vast repertoire of Dscam binding specificities arises from modular interactions of variable Ig domains.
Europe PMC ID: 17889655
Durbin et al. 1988. Int J Cancer Suppl. 2:50-8. PMID: 2450848.
IHC
Durbin et al. 1988. Int J Cancer Suppl. 2:59-66. PMID: 3162446.
ELISA IHC RIA
Production of monoclonal antibodies to placental alkaline phosphatase: preliminary characterisation includes identification of one antibody reactive with routinely fixed histological preparations.
Europe PMC ID: 2450848
Monoclonal antibodies to placental alkaline phosphatase: preclinical evaluation in a human xenograft tumour model of F(ab')2 and Fab fragments.
Europe PMC ID: 3162446
Add a reference
References: 8 entries
Sharp et al. 2010. PLoS One. 5(10):e13130. PMID: 20976190.
IF
Inhibition of cellular protein secretion by norwalk virus nonstructural protein p22 requires a mimic of an endoplasmic reticulum export signal.
Wojtowicz et al. 2007. Cell. 130(6):1134-45. PMID: 17889655.
ELISA
A vast repertoire of Dscam binding specificities arises from modular interactions of variable Ig domains.
Durbin et al. 1988. Int J Cancer Suppl. 2:50-8. PMID: 2450848.
IHC
Durbin et al. 1988. Int J Cancer Suppl. 2:59-66. PMID: 3162446.
ELISA IHC RIA
Production of monoclonal antibodies to placental alkaline phosphatase: preliminary characterisation includes identification of one antibody reactive with routinely fixed histological preparations.
Monoclonal antibodies to placental alkaline phosphatase: preclinical evaluation in a human xenograft tumour model of F(ab')2 and Fab fragments.
Add a reference
