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Catalogue Number 151108
Applications FACS IHC IF
Antigen/Gene or Protein Targets Protein tyrosine phosphatase, receptor type, C (PTPRC, CD45)
Reactivity Human
Relevance Monoclonal antibody capable of analysing subpopulations of hematopoietic and lymphoid cells, and the degree of T cell differentiation. It can be used to identify the effect of CD45 in immune function.

Background and Research Application
Anti-PTPRC (Hle-1) monoclonal antibody binds transmembrane protein tyrosine phosphatase receptor type C (PTPRC), also known as CD45 antigen or leukocyte common antigen (LCA). Anti-PTPRC can be used in a panel of several monoclonal antibodies to characterise tumours. This antibody was created to investigate the similarities between normal thymocyte subpopulations and leukaemia’s of thymic origin (Thy-ALL), and aid with leukaemia diagnosis.
PTPRC is present on all leukocytes. CD45 helps regulate T cell receptor-associated kinases and Janus kinases which transmit signals from cytokine receptors. This regulates the threshold of T cell antigen receptor (TCR) signalling through dephosphorylation of protein tyrosine kinases (e.g. Lck and Fyn). It is only expressed on nucleated hemopoietic cells (~10% of cell surface). CD45 phosphatase activity is required for efficient lymphocyte antigen receptor signal transduction.
CD45 is a positive and negative regulator of Src family members, and can act as a JAK phosphatase, negatively regulating cytokine receptor activation (related to differentiation, proliferation and anti-viral immunity of hemopoietic cells).
Host Mouse
Immunogen Human peripheral blood mononuclear cells.
Immunogen UniProt ID P08575
Subclass IgG1
Molecular Weight (kDa) 180-220
Myeloma Used P3/NS1/1-Ag4.1
Recommended Growing Conditions DMEM + 5% FCS
Strain Balb/c
Notes
RECENTLY UPDATED
Production Details
Purified using multi-step affinity chromatography with protein A.

Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.

Points of Interest
Anti-PTPRC recognises all isoforms of CD45. It reacts strongly with cortical thymocytes, medullary thymus lymphocytes and peripheral lymphoid cells (including T and B cells), but reacts weakly with myeloid and erythroid bone marrow cells. Anti-PTPRC can be used to detect levels of T cell differentiation.

The antibody clone name was called "Hle-1" in Cobbold et al., 1986. Proceedings of the 3rd International Workshop and Conference; Oxford, England. Oxford University Press; 1987. p. 788-803.
Research Area Cardiovascular, Cell Type or Organelle Marker, Cell Signaling & Signal Transduction, Immunology, Neurobiology, Stem Cell Biology

References

There are 7 reference entries for this reagent.

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References: 7 entries

Di Liberto et al. 2016. Clin Transl Gastroenterol. 7(7):e178. PMID: 27388423.

Lee et al. 2004. Int Immunol. 16(8):1109-24. PMID: 15210650.

Gene expression profiles during human CD4+ T cell differentiation.

Europe PMC ID: 15210650

Tchilian et al. 2001. J Immunol. 166(2):1308-13. PMID: 11145714.

A deletion in the gene encoding the CD45 antigen in a patient with SCID.

Europe PMC ID: 11145714

Bradstock et al. 1980. J Natl Cancer Inst. 65(1):33-42. PMID: 6993744.

Subpopulations of normal and leukemic human thymocytes: an analysis with the use of monoclonal antibodies.

Europe PMC ID: 6993744


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References: 7 entries

Di Liberto et al. 2016. Clin Transl Gastroenterol. 7(7):e178. PMID: 27388423.

Lee et al. 2004. Int Immunol. 16(8):1109-24. PMID: 15210650.

Gene expression profiles during human CD4+ T cell differentiation.

Tchilian et al. 2001. J Immunol. 166(2):1308-13. PMID: 11145714.

A deletion in the gene encoding the CD45 antigen in a patient with SCID.

Bradstock et al. 1980. J Natl Cancer Inst. 65(1):33-42. PMID: 6993744.

Subpopulations of normal and leukemic human thymocytes: an analysis with the use of monoclonal antibodies.


Add a reference