Cat. #151108
Anti-CD45 [HLe-1(2D1)]
Cat. #: 151108
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 1-2 weeks
Target: Protein tyrosine phosphatase, receptor type, C (PTPRC, CD45)
Class: Monoclonal
Application: FACS ; IHC ; IF
Reactivity: Human
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Peter Beverley
Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool Details
*FOR RESEARCH USE ONLY
- Name: Anti-CD45 [HLe-1(2D1)]
- Alternate name: Anti-PTPRC
- Clone: HLe-1(2D1)
- Tool type ecom: Antibodies
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Molecular weight: 180-220 kDa
- Strain: Balb/c
- Reactivity: Human
- Host: Mouse
- Application: FACS ; IHC ; IF
- Description: Monoclonal antibody capable of analysing subpopulations of hematopoietic and lymphoid cells, and the degree of T cell differentiation. It can be used to identify the effect of CD45 in immune function.
- Immunogen: Human peripheral blood mononuclear cells.
- Immunogen uniprot id: P08575
- Isotype: IgG1
- Myeloma used: P3/NS1/1-Ag4.1
Target Details
- Target: Protein tyrosine phosphatase, receptor type, C (PTPRC, CD45)
- Molecular weight: 180-220 kDa
- Target background: Anti-PTPRC (Hle-1) monoclonal antibody binds transmembrane protein tyrosine phosphatase receptor type C (PTPRC), also known as CD45 antigen or leukocyte common antigen (LCA). Anti-PTPRC can be used in a panel of several monoclonal antibodies to characterise tumours. This antibody was created to investigate the similarities between normal thymocyte subpopulations and leukaemia's of thymic origin (Thy-ALL), and aid with leukaemia diagnosis. PTPRC is present on all leukocytes. CD45 helps regulate T cell receptor-associated kinases and Janus kinases which transmit signals from cytokine receptors. This regulates the threshold of T cell antigen receptor (TCR) signalling through dephosphorylation of protein tyrosine kinases (e.g. Lck and Fyn). It is only expressed on nucleated hemopoietic cells (~10% of cell surface). CD45 phosphatase activity is required for efficient lymphocyte antigen receptor signal transduction. CD45 is a positive and negative regulator of Src family members, and can act as a JAK phosphatase, negatively regulating cytokine receptor activation (related to differentiation, proliferation and anti-viral immunity of hemopoietic cells).
Applications
- Application: FACS ; IHC ; IF
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: Store at -20° C frozen. Avoid repeated freeze / thaw cycles
- Shipping conditions: Shipping at 4° C
References
- Di Liberto et al. 2016. Clin Transl Gastroenterol. 7(7):e178. PMID: 27388423.
- Lee et al. 2004. Int Immunol. 16(8):1109-24. PMID: 15210650.
- Tchilian et al. 2001. J Immunol. 166(2):1308-13. PMID: 11145714.
- Bradstock et al. 1980. J Natl Cancer Inst. 65(1):33-42. PMID: 6993744.
