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Catalogue Number 151087
Applications IHC IP
Antigen/Gene or Protein Targets Complement receptor 1 (CR1, CD35)
Reactivity Human
Relevance
RECENTLY UPDATED
Monoclonal antibody which binds to a specific epitope in complement receptor 1 without effecting complement activity, with use in CR1 quantitation.

Background and Research Application
CR1 is found on many leucocytes and binds C3b and C4b. E11 detects CR1 on most leukemic monoblasts, granulocytes and may be used to define B cell lymphomas. E11 binds to a 222kDa antigen. This antibody is specific for a binding site in CR1 which is distal from the C3b binding site. As such CR1 activity is not blocked.
CR1 is a cell membrane-bound, monomeric glycoprotein and its primary function is to serve as the cellular receptor for C3b and C4b within the complement system. CR1 is present on neutrophils, eosinophils, monocytes, B-cells, some NK-cells, erythrocytes, myeloid leukaemia’s, follicular dendritic reticulum cells and is negative on basophils.
Anti-CR1 E11 is useful for identifying CR1 on various cells in tissue sections, and for quantitating CR1 on erythrocytes and neutrophils.
Host Mouse
Immunogen Acute monocytic leukaemia cells and normal blood monocytes.
Immunogen UniProt ID
RECENTLY UPDATED
P17927
Subclass
RECENTLY UPDATED
IgG1
Myeloma Used P3/NS1/1-Ag4.1
Recommended Growing Conditions DMEM + 5% FCS
Strain Balb/c
Notes
RECENTLY UPDATED
Production Details
Purified using multi-step affinity chromatography with protein A.

Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.

Points of Interest
All monocytes, neutrophils, lymphocytes and erythrocytes which reacted with anti-CR1 E11 formed EC3b rosettes.

Concentration
1mg/ml as standard
Research Area
RECENTLY UPDATED
Cancer, Immunology

References

There are 8 reference entries for this reagent.

View All References

References: 8 entries
RECENTLY UPDATED

Leucocyte Typing III. Edited by AJ McMichael et al. 1987

Lorenzini et al. 2017. Cell Microbiol. 19(9):. PMID: 28346820.

Fonseca et al. 2016. PLoS One. 11(2):e0149792. PMID: 26914463.

Mahmoudi et al. 2015. Neurobiol Aging. 36(4):1766.e5-1766.e12. PMID: 25666996.

Matthews et al. 2008. Dev Dyn. 237(1):124-31. PMID: 18095340.

Transcription factor onecut3 regulates intrahepatic biliary development in zebrafish.

Europe PMC ID: 18095340

Hogg et al. 1984. Eur J Immunol. 14(3):236-43. PMID: 6368248.

Identification of an anti-monocyte monoclonal antibody that is specific for membrane complement receptor type one (CR1).

Europe PMC ID: 6368248


Add a reference

References: 8 entries
RECENTLY UPDATED

Leucocyte Typing III. Edited by AJ McMichael et al. 1987

Lorenzini et al. 2017. Cell Microbiol. 19(9):. PMID: 28346820.

Fonseca et al. 2016. PLoS One. 11(2):e0149792. PMID: 26914463.

Mahmoudi et al. 2015. Neurobiol Aging. 36(4):1766.e5-1766.e12. PMID: 25666996.

Matthews et al. 2008. Dev Dyn. 237(1):124-31. PMID: 18095340.

Transcription factor onecut3 regulates intrahepatic biliary development in zebrafish.

Hogg et al. 1984. Eur J Immunol. 14(3):236-43. PMID: 6368248.

Identification of an anti-monocyte monoclonal antibody that is specific for membrane complement receptor type one (CR1).


Add a reference