Anti-CR1 [E11]
Invented by Prof Nancy Hogg from Cancer Research UK London Research Institute: Lincoln's Inn Fields
Invented at Cancer Research UK London Research Institute: Lincoln's Inn Fields
- Datasheet
- References (8)
- Inventor Info
Info
| Catalogue Number | 151087 |
| Applications | IHC IP |
| Antigen/Gene or Protein Targets | Complement receptor 1 (CR1, CD35) |
| Reactivity | Human |
| Relevance |
Monoclonal antibody which binds to a specific epitope in complement receptor 1 without effecting complement activity, with use in CR1 quantitation. Background and Research Application CR1 is found on many leucocytes and binds C3b and C4b. E11 detects CR1 on most leukemic monoblasts, granulocytes and may be used to define B cell lymphomas. E11 binds to a 222kDa antigen. This antibody is specific for a binding site in CR1 which is distal from the C3b binding site. As such CR1 activity is not blocked. CR1 is a cell membrane-bound, monomeric glycoprotein and its primary function is to serve as the cellular receptor for C3b and C4b within the complement system. CR1 is present on neutrophils, eosinophils, monocytes, B-cells, some NK-cells, erythrocytes, myeloid leukaemia’s, follicular dendritic reticulum cells and is negative on basophils. Anti-CR1 E11 is useful for identifying CR1 on various cells in tissue sections, and for quantitating CR1 on erythrocytes and neutrophils. |
| Host | Mouse |
| Immunogen | Acute monocytic leukaemia cells and normal blood monocytes. |
| Immunogen UniProt ID | P17927 |
| Subclass | IgG1 |
| Myeloma Used | P3/NS1/1-Ag4.1 |
| Recommended Growing Conditions | DMEM + 5% FCS |
| Strain | Balb/c |
| Notes |
Production Details Purified using multi-step affinity chromatography with protein A. Storage Conditions Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles. Points of Interest All monocytes, neutrophils, lymphocytes and erythrocytes which reacted with anti-CR1 E11 formed EC3b rosettes. Concentration 1mg/ml as standard |
| Research Area | Cancer, Immunology |
References: 8 entries
Leucocyte Typing III. Edited by AJ McMichael et al. 1987
Lorenzini et al. 2017. Cell Microbiol. 19(9):. PMID: 28346820.
Fonseca et al. 2016. PLoS One. 11(2):e0149792. PMID: 26914463.
Mahmoudi et al. 2015. Neurobiol Aging. 36(4):1766.e5-1766.e12. PMID: 25666996.
Matthews et al. 2008. Dev Dyn. 237(1):124-31. PMID: 18095340.
Transcription factor onecut3 regulates intrahepatic biliary development in zebrafish.
Europe PMC ID: 18095340
Hogg et al. 1984. Eur J Immunol. 14(3):236-43. PMID: 6368248.
Identification of an anti-monocyte monoclonal antibody that is specific for membrane complement receptor type one (CR1).
Europe PMC ID: 6368248
Add a reference
References: 8 entries
Leucocyte Typing III. Edited by AJ McMichael et al. 1987
Lorenzini et al. 2017. Cell Microbiol. 19(9):. PMID: 28346820.
Fonseca et al. 2016. PLoS One. 11(2):e0149792. PMID: 26914463.
Mahmoudi et al. 2015. Neurobiol Aging. 36(4):1766.e5-1766.e12. PMID: 25666996.
Matthews et al. 2008. Dev Dyn. 237(1):124-31. PMID: 18095340.
Transcription factor onecut3 regulates intrahepatic biliary development in zebrafish.
Hogg et al. 1984. Eur J Immunol. 14(3):236-43. PMID: 6368248.
Identification of an anti-monocyte monoclonal antibody that is specific for membrane complement receptor type one (CR1).
Add a reference
