Cancer Research Technology
Log in Register
Menu

Mo-pCCy05-Murine Cytokine/Chemokine (T17 Multi-gene) Vector

Info

Vector Type Mo-pCCy05
Antigen/Gene or Protein Targets Murine CCL21, IFN-γ, IL-4, IL-10 and IL-12β
Relevance Useful for research on gene expression, inflammation and inflammatory disease models.
This novel system allows researchers to amplify and compare expression of multiple genes in a quicker and less resource-consuming way. It reduces the variation between individual gene PCR amplification results due to plasmid dilutional errors. Multi-gene plasmid reduces the necessity to maintain multiple plasmid stocks and significantly reduces time and labour costs associated with RT-PCR as only one serial dilution of plasmid is required for all the genes.
Research Area Gene Expression, Immunology
Notes Lowers the necessity to maintain multiple plasmid stocks and significantly minimises time and labour costs associated with RT-PCR.
Only one standard curve dilutional series has to be generated instead of 17 individual ones. Standard curves have been constructed for all 12 chemokines, 3 cytokines and the 2 housekeeping genes. Good linearity of the standard curves was noted for all of the chemokines and cytokines over the range 200 - 2 x 10^8 copies of target.
Sequence analysis confirms the orientation and authenticity of the cloned gene sequences.
All sequences show >98% identity with the DNA sequences deposited in the NCBI database.

References

There are 1 reference entries for this reagent.

View All References

References: 1 entry

Corware et al. 2011. Biomaterials. 32(31):8029-39. PMID: 21807409.


Add a reference

References: 1 entry

Corware et al. 2011. Biomaterials. 32(31):8029-39. PMID: 21807409.


Add a reference

Inventor Information

No inventors are currently linked to this reagent.

Add an inventor