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Anti-PCNA [PC10]

Invented by David Lane at Karolinska Institutet

Info

Catalogue Number 151148
Applications FACS IHC IF IP WB
Antigen/Gene or Protein Targets Proliferating cell nuclear antigen, (PCNA), also known as cyclin or polymerase delta accessory protein
Synonyms Proliferating Cell Nuclear Antigen; DNA Polymerase Delta Auxiliary Protein; ATLD2
Reactivity Human, Insect and Schizosaccharomyces pombe
Relevance PCNA, also known as polymerase delta auxiliary protein, is essential for DNA replication and is involved in DNA excision and mismatch repair pathways. PCNA binds to the CDK inhibitor p21, the structure-specific endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT). PCNA is potentially a therapeutic target in cancer therapy and a useful marker for identifying the proliferation status of tumour tissue (i.e. relevant to prognosis). This antibody can be used for grading of different neoplasms, e.g. astrocytoma. It can be of diagnostic and prognostic value.
Host Mouse
Immunogen Protein A-PCNA fusion obtained from pC2T.
Subclass IgG2a
Molecular Weight (kDa) 36
Myeloma Used Sp2/0-Ag14
Recommended Growing Conditions DMEM + 5% FCS
Notes Recommended for use on formalin-fixed paraffin embedded sections. NEUTRAL ELUTE ONLY.
Research Area Cancer, Cell Cycle, Cell Type or Organelle Marker, Epigenetics & Nuclear Signalling

References: 16 entries

Pacaud et al. 2014. Sci Rep. 4:4230. PMID: 24637615.

The DNMT1/PCNA/UHRF1 disruption induces tumorigenesis characterized by similar genetic and epigenetic signatures.

Europe PMC ID: 24637615

Kuang et al. 2013. World J Gastroenterol. 19(39):6637-44. PMID: 24151393.

Long-term potentiation promotes proliferation/survival and neuronal differentiation of neural stem/progenitor cells.

Europe PMC ID: 24146937

Expression and significance of Musashi-1 in gastric cancer and precancerous lesions.

Europe PMC ID: 24151393

Cho et al. 2013. PLoS One. 8(10):e76860. PMID: 24146937.

Landberg et al. 1991. Cancer Res. 51(17):4570-4. PMID: 1678682.

Antibodies to proliferating cell nuclear antigen as S-phase probes in flow cytometric cell cycle analysis.

Europe PMC ID: 1678682

Yu et al. 1991. Histopathology. 19(1):29-33. PMID: 1680785.

Woods et al. 1991. Histopathology. 19(1):21-7. PMID: 1680784.

Haemangiopericytomas: the prognostic value of immunohistochemical staining with a monoclonal antibody to proliferating cell nuclear antigen (PCNA).

Europe PMC ID: 1680785

The assessment of proliferating cell nuclear antigen (PCNA) immunostaining in primary gastrointestinal lymphomas and its relationship to histological grade, S+G2+M phase fraction (flow cytometric analysis) and prognosis.

Europe PMC ID: 1680784

Hall et al. 1990. J Pathol. 162(4):285-94. PMID: 1981239.

Proliferating cell nuclear antigen (PCNA) immunolocalization in paraffin sections: an index of cell proliferation with evidence of deregulated expression in some neoplasms.

Europe PMC ID: 1981239

Waseem et al. 1990. J Cell Sci. 96 ( Pt 1):121-9. PMID: 1695635.

Monoclonal antibody analysis of the proliferating cell nuclear antigen (PCNA). Structural conservation and the detection of a nucleolar form.

Europe PMC ID: 1695635


Add a reference

References: 16 entries

Pacaud et al. 2014. Sci Rep. 4:4230. PMID: 24637615.

The DNMT1/PCNA/UHRF1 disruption induces tumorigenesis characterized by similar genetic and epigenetic signatures.

Europe PMC ID: 24637615

Kuang et al. 2013. World J Gastroenterol. 19(39):6637-44. PMID: 24151393.

Long-term potentiation promotes proliferation/survival and neuronal differentiation of neural stem/progenitor cells.

Europe PMC ID: 24146937

Expression and significance of Musashi-1 in gastric cancer and precancerous lesions.

Europe PMC ID: 24151393

Cho et al. 2013. PLoS One. 8(10):e76860. PMID: 24146937.

Landberg et al. 1991. Cancer Res. 51(17):4570-4. PMID: 1678682.

Antibodies to proliferating cell nuclear antigen as S-phase probes in flow cytometric cell cycle analysis.

Europe PMC ID: 1678682

Yu et al. 1991. Histopathology. 19(1):29-33. PMID: 1680785.

Woods et al. 1991. Histopathology. 19(1):21-7. PMID: 1680784.

Haemangiopericytomas: the prognostic value of immunohistochemical staining with a monoclonal antibody to proliferating cell nuclear antigen (PCNA).

Europe PMC ID: 1680785

The assessment of proliferating cell nuclear antigen (PCNA) immunostaining in primary gastrointestinal lymphomas and its relationship to histological grade, S+G2+M phase fraction (flow cytometric analysis) and prognosis.

Europe PMC ID: 1680784

Hall et al. 1990. J Pathol. 162(4):285-94. PMID: 1981239.

Proliferating cell nuclear antigen (PCNA) immunolocalization in paraffin sections: an index of cell proliferation with evidence of deregulated expression in some neoplasms.

Europe PMC ID: 1981239

Waseem et al. 1990. J Cell Sci. 96 ( Pt 1):121-9. PMID: 1695635.

Monoclonal antibody analysis of the proliferating cell nuclear antigen (PCNA). Structural conservation and the detection of a nucleolar form.

Europe PMC ID: 1695635


Add a reference

References: 16 entries

Pacaud et al. 2014. Sci Rep. 4:4230. PMID: 24637615.

The DNMT1/PCNA/UHRF1 disruption induces tumorigenesis characterized by similar genetic and epigenetic signatures.

Kuang et al. 2013. World J Gastroenterol. 19(39):6637-44. PMID: 24151393.

Long-term potentiation promotes proliferation/survival and neuronal differentiation of neural stem/progenitor cells.

Expression and significance of Musashi-1 in gastric cancer and precancerous lesions.

Cho et al. 2013. PLoS One. 8(10):e76860. PMID: 24146937.

Landberg et al. 1991. Cancer Res. 51(17):4570-4. PMID: 1678682.

Antibodies to proliferating cell nuclear antigen as S-phase probes in flow cytometric cell cycle analysis.

Yu et al. 1991. Histopathology. 19(1):29-33. PMID: 1680785.

Woods et al. 1991. Histopathology. 19(1):21-7. PMID: 1680784.

Haemangiopericytomas: the prognostic value of immunohistochemical staining with a monoclonal antibody to proliferating cell nuclear antigen (PCNA).

The assessment of proliferating cell nuclear antigen (PCNA) immunostaining in primary gastrointestinal lymphomas and its relationship to histological grade, S+G2+M phase fraction (flow cytometric analysis) and prognosis.

Hall et al. 1990. J Pathol. 162(4):285-94. PMID: 1981239.

Proliferating cell nuclear antigen (PCNA) immunolocalization in paraffin sections: an index of cell proliferation with evidence of deregulated expression in some neoplasms.

Waseem et al. 1990. J Cell Sci. 96 ( Pt 1):121-9. PMID: 1695635.

Monoclonal antibody analysis of the proliferating cell nuclear antigen (PCNA). Structural conservation and the detection of a nucleolar form.


Add a reference


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