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Monoclonal Antibodies

Anti-PCNA [PC10]

Invented by David Lane from Karolinska Institutet
Invented at Cancer Research UK London Research Institute: Clare Hall Laboratories
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Adapted from PLoS One. 8(10):e76860. PMID: 24146937 Figure. NMDAR-dependent LTP enhances proliferation of NPCs in the DG. (B) LTP increases the total numbers of PCNA-positive immature neurons in the DG. Representative images from coronal sections from non-LTP (0.05 Hz+ saline) and LTP-induced (LTP) rats co-stained with PCNA (red) and DAPI (blue) in the DG area. Examples of doubled-stained cells in boxed areas were shown in higher magnification on the right panels. Bar graph on the right summarizes data from various groups, suggesting enhancement of proliferation by LTP induction. Note CPP pretreatment alone (0.05 Hz+CPP) has no effect on intrinsic proliferation, but prevented LTP-promoted NPC proliferation (CPP+sTPS). **p<0.01, n = 8 or 9. Statistical analysis was performed with One Way ANOVA. Post hoc testing revealed a significant difference between the LTP group and other three groups. Data are mean ± SEM. significantly different from each other.
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Catalogue Number 151148
Applications FACS IHC IF IP WB
Antigen/Gene or Protein Targets Proliferating cell nuclear antigen, (PCNA), also known as cyclin or polymerase delta accessory protein
Synonyms Proliferating Cell Nuclear Antigen; DNA Polymerase Delta Auxiliary Protein; ATLD2
Reactivity Human, Insect and Schizosaccharomyces pombe
Relevance PCNA, also known as polymerase delta auxiliary protein, is essential for DNA replication and is involved in DNA excision and mismatch repair pathways. PCNA binds to the CDK inhibitor p21, the structure-specific endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT). PCNA is potentially a therapeutic target in cancer therapy and a useful marker for identifying the proliferation status of tumour tissue (i.e. relevant to prognosis). This antibody can be used for grading of different neoplasms, e.g. astrocytoma. It can be of diagnostic and prognostic value.
Host Mouse
Immunogen Protein A-PCNA fusion obtained from pC2T.
Subclass IgG2a
Molecular Weight (kDa) 36
Myeloma Used Sp2/0-Ag14
Recommended Growing Conditions DMEM + 5% FCS
Notes Recommended for use on formalin-fixed paraffin embedded sections. NEUTRAL ELUTE ONLY.
Research Area Cancer, Cell Cycle, Cell Type or Organelle Marker, Epigenetics & Nuclear Signalling

References

There are 16 reference entries for this reagent.

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References: 16 entries

Pacaud et al. 2014. Sci Rep. 4:4230. PMID: 24637615.

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The DNMT1/PCNA/UHRF1 disruption induces tumorigenesis characterized by similar genetic and epigenetic signatures.

Europe PMC ID: 24637615

View  

Kuang et al. 2013. World J Gastroenterol. 19(39):6637-44. PMID: 24151393.

View  

Long-term potentiation promotes proliferation/survival and neuronal differentiation of neural stem/progenitor cells.

Europe PMC ID: 24146937

View  

Expression and significance of Musashi-1 in gastric cancer and precancerous lesions.

Europe PMC ID: 24151393

View  

Cho et al. 2013. PLoS One. 8(10):e76860. PMID: 24146937.

View  

Landberg et al. 1991. Cancer Res. 51(17):4570-4. PMID: 1678682.

View  

Antibodies to proliferating cell nuclear antigen as S-phase probes in flow cytometric cell cycle analysis.

Europe PMC ID: 1678682

View  

Yu et al. 1991. Histopathology. 19(1):29-33. PMID: 1680785.

View  

Woods et al. 1991. Histopathology. 19(1):21-7. PMID: 1680784.

View  

Haemangiopericytomas: the prognostic value of immunohistochemical staining with a monoclonal antibody to proliferating cell nuclear antigen (PCNA).

Europe PMC ID: 1680785

View  

The assessment of proliferating cell nuclear antigen (PCNA) immunostaining in primary gastrointestinal lymphomas and its relationship to histological grade, S+G2+M phase fraction (flow cytometric analysis) and prognosis.

Europe PMC ID: 1680784

View  

Hall et al. 1990. J Pathol. 162(4):285-94. PMID: 1981239.

View  

Proliferating cell nuclear antigen (PCNA) immunolocalization in paraffin sections: an index of cell proliferation with evidence of deregulated expression in some neoplasms.

Europe PMC ID: 1981239

View  

Waseem et al. 1990. J Cell Sci. 96 ( Pt 1):121-9. PMID: 1695635.

View  

Monoclonal antibody analysis of the proliferating cell nuclear antigen (PCNA). Structural conservation and the detection of a nucleolar form.

Europe PMC ID: 1695635

View  

Add a reference

References: 16 entries

Pacaud et al. 2014. Sci Rep. 4:4230. PMID: 24637615.

View  

The DNMT1/PCNA/UHRF1 disruption induces tumorigenesis characterized by similar genetic and epigenetic signatures.

View  

Kuang et al. 2013. World J Gastroenterol. 19(39):6637-44. PMID: 24151393.

View  

Long-term potentiation promotes proliferation/survival and neuronal differentiation of neural stem/progenitor cells.

View  

Expression and significance of Musashi-1 in gastric cancer and precancerous lesions.

View  

Cho et al. 2013. PLoS One. 8(10):e76860. PMID: 24146937.

View  

Landberg et al. 1991. Cancer Res. 51(17):4570-4. PMID: 1678682.

View  

Antibodies to proliferating cell nuclear antigen as S-phase probes in flow cytometric cell cycle analysis.

View  

Yu et al. 1991. Histopathology. 19(1):29-33. PMID: 1680785.

View  

Woods et al. 1991. Histopathology. 19(1):21-7. PMID: 1680784.

View  

Haemangiopericytomas: the prognostic value of immunohistochemical staining with a monoclonal antibody to proliferating cell nuclear antigen (PCNA).

View  

The assessment of proliferating cell nuclear antigen (PCNA) immunostaining in primary gastrointestinal lymphomas and its relationship to histological grade, S+G2+M phase fraction (flow cytometric analysis) and prognosis.

View  

Hall et al. 1990. J Pathol. 162(4):285-94. PMID: 1981239.

View  

Proliferating cell nuclear antigen (PCNA) immunolocalization in paraffin sections: an index of cell proliferation with evidence of deregulated expression in some neoplasms.

View  

Waseem et al. 1990. J Cell Sci. 96 ( Pt 1):121-9. PMID: 1695635.

View  

Monoclonal antibody analysis of the proliferating cell nuclear antigen (PCNA). Structural conservation and the detection of a nucleolar form.

View  

Add a reference

Inventor Information