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KARPAS 25 Cell Line

Invented by Abraham Karpas

Info

Antigen/Gene or Protein Targets Human acute plasma cell leukaemia
Host Human
Tissue Blood
Disease Keywords Cancer; haemotology; leukaemia
Relevance The KARPAS 25 cell line is a Human acute plasma cell leukaemia cell line useful in oncology research.
Production Details Established in 1989 from the pleural effusion of a 27-year old woman diagnosed with acute plasma cell leukaemia who failed to respond to treatment and died within 3 weeks.
Research Area Cancer, Drug Discovery & Development
Growth/Phenotype Keywords Round to polygonal cells growing singly in suspension; Hyperdiploid with complex aberrant chromosomes
Recommended Growing Conditions Doubling time approximately 30 hours. Cells grow singly in suspension. Maintain cultures between 5-20 x 105 cells/ml; 5% CO2; 37°C. Thaw ampoule and perform a cell count, spin out the DMSO and resuspend in an appropriate volume of media to give a cell concentration of 5 x 105 cells/ml. At subculture perform a cell count and maintain cultures between 5-20 x 105cells/ml, this tends to equate to a 1:3 split every 2-3 days.Culture medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)
Cellosaurus ID CVCL_2540

References: 1 entry

Karpas et al. 2001. Proc Natl Acad Sci U S A. 98(4):1799-804. PMID: 11172031.


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References: 1 entry

Karpas et al. 2001. Proc Natl Acad Sci U S A. 98(4):1799-804. PMID: 11172031.


Add a reference

References: 1 entry

Karpas et al. 2001. Proc Natl Acad Sci U S A. 98(4):1799-804. PMID: 11172031.


Add a reference


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