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Antigen/Gene or Protein Targets
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Human acute plasma cell leukaemia
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Host
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Human
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Tissue
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Blood
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Disease Keywords
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Cancer; haemotology; leukaemia
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Relevance
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The KARPAS 25 cell line is a Human acute plasma cell leukaemia cell line useful in oncology research.
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Production Details
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Established in 1989 from the pleural effusion of a 27-year old woman diagnosed with acute plasma cell leukaemia who failed to respond to treatment and died within 3 weeks.
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Research Area
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Cancer, Drug Discovery & Development
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Growth/Phenotype Keywords
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Round to polygonal cells growing singly in suspension; Hyperdiploid with complex aberrant chromosomes
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Recommended Growing Conditions
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Doubling time approximately 30 hours. Cells grow singly in suspension. Maintain cultures between 5-20 x 105 cells/ml; 5% CO2; 37°C. Thaw ampoule and perform a cell count, spin out the DMSO and resuspend in an appropriate volume of media to give a cell concentration of 5 x 105 cells/ml. At subculture perform a cell count and maintain cultures between 5-20 x 105cells/ml, this tends to equate to a 1:3 split every 2-3 days.Culture medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)
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Cellosaurus ID
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CVCL_2540
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