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KARPAS 422 Cell Line

Invented by Abraham Karpas

Info

Antigen/Gene or Protein Targets Human B cell non-Hodgkin lymphoma
Host Human
Tissue Blood
Disease Keywords Cancer; haemotology; Lymphoma B-cell
Relevance The KARPAS 422 cell line is a Human B-Cell Non-Hodgkin Lymphoma cell line useful in oncology research. Carries t(14;18) IGH-BCL2 fusion gene (break point in major breakpoint region, MBR).
Production Details Established from a pleural effusion of 73 year-old woman diagnosed with B-cell non-Hodgkin lymphoma (intra-abdominal, diffuse large cell lymphoma, refractory, terminal). Carries t(14;18) IGH-BCL2 fusion gene (break point in major breakpoint region, MBR).
Research Area Cancer, Drug Discovery & Development
Growth/Phenotype Keywords Round polygonal cells growing singly or in small clusters
Recommended Growing Conditions If starting from a frozen ampoule the cryoprotectant should be removed. Add thawed cells to a conical based centrifuge tube e.g. 15ml tube; slowly add 4 ml of culture medium to the tube. Take a sample of the cell suspension, e.g. 100μl, to count cells. Centrifuge the cell suspension at low speed i.e. 100 - 150 x g for a maximum of 5 minutes. Remove medium and resuspend the cell pellet at a density of 5 x 100,000 cells/ml in fresh medium containing 20% serum. Incubate flask at 37°C; 5 - 7% CO2. Check daily. Keep flask in a vertical position until the cells reach the exponential phase of growth. This can take up to 7 days. Once the culture is established the serum concentration can be reduced to 10%.Maintain cultures between 5 - 20 x100,000 cells/ml by splitting saturated cultures 1:2 every 2-4 days; 5% CO2; 37°C. Doubling time approximately 60-90 hours. Round polygonal cells growing singly or in small clusters in suspension.Culture medium: RPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).
Cellosaurus ID CVCL_1325

References: 1 entry

Howell et al. 1990. Bone Marrow Transplant. 5(1):66-7. PMID: 2297593.


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References: 1 entry

Howell et al. 1990. Bone Marrow Transplant. 5(1):66-7. PMID: 2297593.


Add a reference

References: 1 entry

Howell et al. 1990. Bone Marrow Transplant. 5(1):66-7. PMID: 2297593.


Add a reference


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