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Antigen/Gene or Protein Targets
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Human B cell non-Hodgkin lymphoma
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Host
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Human
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Tissue
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Blood
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Disease Keywords
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Cancer; haemotology; Lymphoma B-cell
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Relevance
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The KARPAS 422 cell line is a Human B-Cell Non-Hodgkin Lymphoma cell line useful in oncology research. Carries t(14;18) IGH-BCL2 fusion gene (break point in major breakpoint region, MBR).
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Production Details
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Established from a pleural effusion of 73 year-old woman diagnosed with B-cell non-Hodgkin lymphoma (intra-abdominal, diffuse large cell lymphoma, refractory, terminal). Carries t(14;18) IGH-BCL2 fusion gene (break point in major breakpoint region, MBR).
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Research Area
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Cancer, Drug Discovery & Development
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Growth/Phenotype Keywords
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Round polygonal cells growing singly or in small clusters
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Recommended Growing Conditions
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If starting from a frozen ampoule the cryoprotectant should be removed. Add thawed cells to a conical based centrifuge tube e.g. 15ml tube; slowly add 4 ml of culture medium to the tube. Take a sample of the cell suspension, e.g. 100μl, to count cells. Centrifuge the cell suspension at low speed i.e. 100 - 150 x g for a maximum of 5 minutes. Remove medium and resuspend the cell pellet at a density of 5 x 100,000 cells/ml in fresh medium containing 20% serum. Incubate flask at 37°C; 5 - 7% CO2. Check daily. Keep flask in a vertical position until the cells reach the exponential phase of growth. This can take up to 7 days. Once the culture is established the serum concentration can be reduced to 10%.Maintain cultures between 5 - 20 x100,000 cells/ml by splitting saturated cultures 1:2 every 2-4 days; 5% CO2; 37°C. Doubling time approximately 60-90 hours. Round polygonal cells growing singly or in small clusters in suspension.Culture medium: RPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).
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Cellosaurus ID
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CVCL_1325
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