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KARPAS 299 Cell Line

Invented by Abraham Karpas at University of Cambridge

Info

Antigen/Gene or Protein Targets CD30+ anaplastic large cell lymphoma (ALCL); cells carry the NPM-ALK fusion gene
Host Human
Tissue Blood
Disease Keywords Cancer; haemotology; Lymphoma Non-Hodgkins
Relevance KARPAS 299 cell line which carries the NPM-ALK fusion gene.

Background and Research Application
The KARPAS 299 cell line is a Human Non-Hodgkin's Ki-positive Large Cell Lymphoma cell line useful in oncology research. The cells carry the NPM-ALK fusion gene. This cell line is derived from a patient with anaplastic large cell lymphoma, ALK positive.
Production Details A human T cell lymphoma cell line established from the peripheral blood of a 25-year-old man with T cell non-Hodgkin's lymphoma in 1986; now classed as CD30+ anaplastic large cell lymphoma (ALCL); cells carry the NPM-ALK fusion gene.
Research Area Cancer, Drug Discovery & Development
Growth/Phenotype Keywords Lymphoblastoid; Hypodiploid
Recommended Growing Conditions Maintain cultures between 0.5 - 2 x1000,000 cells/ml; 5% CO2; 37°C. Doubling time approximately 30 hours. Split saturated culture 1:3 every 2-3 days. Round to polygonal cells growing singly or in small clusters in suspension.Culture medium: RPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS). For slower growth use 10% or 5% FBS.
Notes Concentration
Vial has between 1-5 million cells as standard, however this may vary.
Cellosaurus ID CVCL_1324

References

There are 9 reference entries for this reagent.

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References: 9 entries

Drexler et al. 2004. Leukemia. 18(10):1569-71. PMID: 15356658.

Fischer et al. 1988. Blood. 72(1):234-40. PMID: 3260522.

Mason et al. 1990. Br J Haematol. 74(2):161-8. PMID: 2156548.

Nusinow et al. 2020. Cell. 180(2):387-402.e16. PMID: 31978347.

Ghandi et al. 2019. Nature. 569(7757):503-508. PMID: 31068700.

Dutil et al. 2019. Cancer Res. 79(7):1263-1273. PMID: 30894373.

Tan et al. 2018. BMC Cancer. 18(1):940. PMID: 30285677.

Krumbholz et al. 2018. Oncotarget. 9(41):26543-26555. PMID: 29899875.

Li et al. 2017. Cancer Cell. 31(2):225-239. PMID: 28196595.


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References: 9 entries

Drexler et al. 2004. Leukemia. 18(10):1569-71. PMID: 15356658.

Fischer et al. 1988. Blood. 72(1):234-40. PMID: 3260522.

Mason et al. 1990. Br J Haematol. 74(2):161-8. PMID: 2156548.

Nusinow et al. 2020. Cell. 180(2):387-402.e16. PMID: 31978347.

Ghandi et al. 2019. Nature. 569(7757):503-508. PMID: 31068700.

Dutil et al. 2019. Cancer Res. 79(7):1263-1273. PMID: 30894373.

Tan et al. 2018. BMC Cancer. 18(1):940. PMID: 30285677.

Krumbholz et al. 2018. Oncotarget. 9(41):26543-26555. PMID: 29899875.

Li et al. 2017. Cancer Cell. 31(2):225-239. PMID: 28196595.


Add a reference

Inventor Information