Log in Register
Menu

KARPAS 299 Cell Line

Invented by Abraham Karpas

Info

Antigen/Gene or Protein Targets CD30+ anaplastic large cell lymphoma (ALCL); cells carry the NPM-ALK fusion gene
Host Human
Tissue Blood
Disease Keywords Cancer; haemotology; Lymphoma Non-Hodgkins
Relevance The KARPAS 299 cell line is a Human Non-Hodgkin's Ki-positive Large Cell Lymphoma cell line useful in oncology research. The cells carry the NPM-ALK fusion gene.
Production Details A human T cell lymphoma cell line established from the peripheral blood of a 25-year-old man with T cell non-Hodgkin's lymphoma in 1986; now classed as CD30+ anaplastic large cell lymphoma (ALCL); cells carry the NPM-ALK fusion gene.
Research Area Cancer, Drug Discovery & Development
Growth/Phenotype Keywords Lymphoblastoid; Hypodiploid
Recommended Growing Conditions Maintain cultures between 0.5 - 2 x1000,000 cells/ml; 5% CO2; 37°C. Doubling time approximately 30 hours. Split saturated culture 1:3 every 2-3 days. Round to polygonal cells growing singly or in small clusters in suspension.Culture medium: RPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS). For slower growth use 10% or 5% FBS.
Cellosaurus ID CVCL_1324

References: 3 entries

Drexler et al. 2004. Leukemia. 18(10):1569-71. PMID: 15356658.

Fischer et al. 1988. Blood. 72(1):234-40. PMID: 3260522.

Mason et al. 1990. Br J Haematol. 74(2):161-8. PMID: 2156548.


Add a reference

References: 3 entries

Drexler et al. 2004. Leukemia. 18(10):1569-71. PMID: 15356658.

Fischer et al. 1988. Blood. 72(1):234-40. PMID: 3260522.

Mason et al. 1990. Br J Haematol. 74(2):161-8. PMID: 2156548.


Add a reference

References: 3 entries

Drexler et al. 2004. Leukemia. 18(10):1569-71. PMID: 15356658.

Fischer et al. 1988. Blood. 72(1):234-40. PMID: 3260522.

Mason et al. 1990. Br J Haematol. 74(2):161-8. PMID: 2156548.


Add a reference


This reagent does not have any reviews at the moment.