- Datasheet
- References (9)
- Inventor Info
Info
Antigen/Gene or Protein Targets | CD30+ anaplastic large cell lymphoma (ALCL); cells carry the NPM-ALK fusion gene |
Host | Human |
Tissue | Blood |
Disease Keywords | Cancer; haemotology; Lymphoma Non-Hodgkins |
Relevance |
KARPAS 299 cell line which carries the NPM-ALK fusion gene. Background and Research Application The KARPAS 299 cell line is a Human Non-Hodgkin's Ki-positive Large Cell Lymphoma cell line useful in oncology research. The cells carry the NPM-ALK fusion gene. This cell line is derived from a patient with anaplastic large cell lymphoma, ALK positive. |
Production Details | A human T cell lymphoma cell line established from the peripheral blood of a 25-year-old man with T cell non-Hodgkin's lymphoma in 1986; now classed as CD30+ anaplastic large cell lymphoma (ALCL); cells carry the NPM-ALK fusion gene. |
Research Area | Cancer, Drug Discovery & Development |
Growth/Phenotype Keywords | Lymphoblastoid; Hypodiploid |
Recommended Growing Conditions | Maintain cultures between 0.5 - 2 x1000,000 cells/ml; 5% CO2; 37°C. Doubling time approximately 30 hours. Split saturated culture 1:3 every 2-3 days. Round to polygonal cells growing singly or in small clusters in suspension.Culture medium: RPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS). For slower growth use 10% or 5% FBS. |
Notes |
Concentration Vial has between 1-5 million cells as standard, however this may vary. |
Cellosaurus ID | CVCL_1324 |
References: 9 entries
Drexler et al. 2004. Leukemia. 18(10):1569-71. PMID: 15356658.
Fischer et al. 1988. Blood. 72(1):234-40. PMID: 3260522.
Mason et al. 1990. Br J Haematol. 74(2):161-8. PMID: 2156548.
Nusinow et al. 2020. Cell. 180(2):387-402.e16. PMID: 31978347.
Ghandi et al. 2019. Nature. 569(7757):503-508. PMID: 31068700.
Dutil et al. 2019. Cancer Res. 79(7):1263-1273. PMID: 30894373.
Tan et al. 2018. BMC Cancer. 18(1):940. PMID: 30285677.
Krumbholz et al. 2018. Oncotarget. 9(41):26543-26555. PMID: 29899875.
Li et al. 2017. Cancer Cell. 31(2):225-239. PMID: 28196595.
Add a reference
References: 9 entries
Drexler et al. 2004. Leukemia. 18(10):1569-71. PMID: 15356658.
Fischer et al. 1988. Blood. 72(1):234-40. PMID: 3260522.
Mason et al. 1990. Br J Haematol. 74(2):161-8. PMID: 2156548.
Nusinow et al. 2020. Cell. 180(2):387-402.e16. PMID: 31978347.
Ghandi et al. 2019. Nature. 569(7757):503-508. PMID: 31068700.
Dutil et al. 2019. Cancer Res. 79(7):1263-1273. PMID: 30894373.
Tan et al. 2018. BMC Cancer. 18(1):940. PMID: 30285677.
Krumbholz et al. 2018. Oncotarget. 9(41):26543-26555. PMID: 29899875.
Li et al. 2017. Cancer Cell. 31(2):225-239. PMID: 28196595.
Add a reference
Inventor Information
Inventors
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Abraham Karpas |