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Monoclonal Antibodies

Anti-PKCa [MC5]

Invented at Cancer Research UK London Research Institute: Lincoln's Inn Fields
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The expression of PKCα protein in three esophageal cancer cells and its statistical analysis map. Compared with HEEC, *p<0.01. Adapted from Gu et al, Biol. Pharm. Bull. 42, 573–579 (2019)
Oral lactate administration regulated protein kinase C (PKC) expression in the hypothalamic area. (a) Both the lactate 100 and 250 groups significantly upregulated the mean PKC-IR cell density in the hypothalamus (n = 6). (b) Representative images of all groups for PKC+ cell expression in hypothalamic region. PKC density was markedly increased in both lactate groups compared to the control group (the scale bar represents 200 µm). Values are presented as mean ± SEM. ** show differences between the normal and control groups, while ## and ### show significant differences between the control group and treatment groups. ## p < 0.01 and ### p < 0.001. The p-value of the test was <0.001 by one-way ANOVA, followed by a post hoc LSD test. Adapted from Shaif et al Biomedicines 2018, 6, 108; doi:10.3390/biomedicines6040108
Increased occurrence of ectopic ribbon synapses in CAST KO and dKO retinae. (A and B) Representative confocal images of immunolabeled retinal sections, showing distribution of presynaptic proteins (RIBEYE in A, vGluT1 in B; green) with respect to BCs (PKC; magenta; A) and HCs (calbindin; magenta; B). Ectopically localized synapses in the ONL of CAST KO and dKO retinae (indicated by white arrows). Bars, 50 µm in A; 20 µm in B. Adapted from Hagiwara et al, J. Cell Biol. 2018 Vol. 217 No. 11 3993–4006
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Catalogue Number 151131
Applications ELISA FACS IHC IF IP RIA WB
Antigen/Gene or Protein Targets Protein Kinase C alpha
Reactivity Bovine
Relevance Protein Kinase C is a family of serine/threonine protein kinases that are activated by Ca2+, diacylglycerol and membrane phospholipids (phosphatidylserine). MC5 blocks partial proteolysis of PKC by trypsin in vitro. Fab fragment inhibits phorbol-ester-induced down-regulation of PKC in a rodent gloma cell line.
Host Mouse
Immunogen Purified bovine brain protein kinase C
Subclass IgG2a
Molecular Weight (kDa) 80
Myeloma Used P3X63Ag8
Recommended Growing Conditions DMEM + 5% FCS
Strain Balb/c
Research Area Cancer, Cardiovascular, Cell Signaling & Signal Transduction

References

There are 3 reference entries for this reagent.

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References: 3 entries

Kim et al. 2008. Nature. 452(7186):478-82. PMID: 18368118.

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Ng et al. 1999. EMBO J. 18(14):3909-23. PMID: 10406796.

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Young et al. 1988. Eur J Biochem. 173(1):247-52. PMID: 2451608.

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References: 3 entries

Kim et al. 2008. Nature. 452(7186):478-82. PMID: 18368118.

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Ng et al. 1999. EMBO J. 18(14):3909-23. PMID: 10406796.

View  

Young et al. 1988. Eur J Biochem. 173(1):247-52. PMID: 2451608.

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