Anti-Integrin b3 [MHF4]
Invented at Queen Mary University of London
- Datasheet
- References (6)
- Inventor Info
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![Adapted from Ballana E, Pauls E, Clotet B, Perron-Sierra F, Tucker GC, Esté JA. β5 integrin is the major contributor to the αVintegrin-mediated blockade of HIV-1 replication. J Immunol. 2011 Jan 1;186(1):464-70. doi: 10.4049/jimmunol.1002693. Epub 2010 Nov 22. PMID: 21098231. Downregulation of αv-coupled β integrins did not affect cell shape or adhesion. Immunofluorescence of MDMs mock-transfected or treated with siRNAs [nontargeting siRNA, siβ3 (A) and siβ5 (B)]. Cells were immunostained for the corresponding β integrin (green) and counterstained for F-actin with phalloidin (red). No changes in cell shape and adhesion were observed as a consequence of β-integrin downregulation.](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,q_auto/3901dd3d-356a-46fb-893c-38851310f93e.jpg)
![Adapted from Ballana E, Pauls E, Clotet B, Perron-Sierra F, Tucker GC, Esté JA. β5 integrin is the major contributor to the αVintegrin-mediated blockade of HIV-1 replication. J Immunol. 2011 Jan 1;186(1):464-70. doi: 10.4049/jimmunol.1002693. Epub 2010 Nov 22. PMID: 21098231. Downregulation of αv-coupled β integrins did not affect cell shape or adhesion. Immunofluorescence of MDMs mock-transfected or treated with siRNAs [nontargeting siRNA, siβ3 (A) and siβ5 (B)]. Cells were immunostained for the corresponding β integrin (green) and counterstained for F-actin with phalloidin (red). No changes in cell shape and adhesion were observed as a consequence of β-integrin downregulation.](https://res.cloudinary.com/ximbio/image/upload/c_limit,fl_lossy,h_282,q_auto,w_368/3901dd3d-356a-46fb-893c-38851310f93e.jpg)
![Image thumbnail for Anti-Integrin b3 [MHF4]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/42ca4436-492e-4821-8e1f-f01e752671c8.jpg)
![Image thumbnail for Anti-Integrin b3 [MHF4]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/d73eb24c-f9c5-412f-a786-d3ea4f6c322d.jpg)
![Image thumbnail for Anti-Integrin b3 [MHF4]](https://res.cloudinary.com/ximbio/image/upload/c_fit,fl_lossy,h_45,q_auto/3901dd3d-356a-46fb-893c-38851310f93e.jpg)
| Catalogue Number | 151133 |
| Applications | FACS IHC IF IP |
| Antigen/Gene or Protein Targets | Integrin ß3 chain (CD61) |
| Reactivity | Human and Rat |
| Relevance | Integrins are heterodimeric cell surface receptors composed of alpha and beta subunits, which mediate cell-cell and cell-extracellular matrix attachments. Aberrant integrin expression has been found in many epithelial tumours. Changes in integrin expression have been shown to be important for the growth and early metastatic capacity of melanoma cells. Integrin b3 is expressed in osteoclasts, megacaryocytes and platelets. MHF4 may be used to identify osteoclasts in tissue sections and bone cell suspensions. The MHF4 epitope is different to the F11 epitope. |
| Host | Mouse |
| Immunogen | A bone cell suspension containing osteoclasts, osteoblasts and bone marrow cells obtained from the long bones of newborn Sprague Dawley rats. |
| Subclass | IgG1 |
| Molecular Weight (kDa) | 118/100 |
| Recommended Growing Conditions | DMEM + 5% FCS |
| Strain | Balb/c |
| Notes | A heterodimeric molecule with a characteristic shift in apparent molecular mass on reduction (137/78 kDa nonreduced; 118/100 kDa reduced). |
| Research Area | Adhesion, Cardiovascular, Cell Signaling & Signal Transduction |
References: 6 entries
Human Adenovirus Type 37 Uses αVβ1 and α3β1 Integrins for Infection of Human Corneal Cells.
Europe PMC ID: 27974569
Storm et al. 2016. J Virol. :. PMID: 27974569.
FACS IHC
Ballana et al. 2011. J Immunol. 186(1):464-70. PMID: 21098231.
β5 integrin is the major contributor to the αVintegrin-mediated blockade of HIV-1 replication.
Europe PMC ID: 21098231
Helfrich et al. 1992. J Bone Miner Res. 7(3):345-51. PMID: 1585837.
Integrins on rat osteoclasts: characterization of two monoclonal antibodies (F4 and F11) to rat beta 3.
Europe PMC ID: 1585837
Add a reference
References: 6 entries
Human Adenovirus Type 37 Uses αVβ1 and α3β1 Integrins for Infection of Human Corneal Cells.
Storm et al. 2016. J Virol. :. PMID: 27974569.
FACS IHC
Ballana et al. 2011. J Immunol. 186(1):464-70. PMID: 21098231.
β5 integrin is the major contributor to the αVintegrin-mediated blockade of HIV-1 replication.
Helfrich et al. 1992. J Bone Miner Res. 7(3):345-51. PMID: 1585837.
Integrins on rat osteoclasts: characterization of two monoclonal antibodies (F4 and F11) to rat beta 3.
Add a reference
