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Vectors

pcDNA5/FRT/TO SBBP-CDK1 WT (2007) Vector

Invented by Dr Jonathon Pines from Gurdon Institute
Invented at University of Cambridge
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Antigen/Gene or Protein Targets CDK1
Relevance Species: H. sapiens (human)
Promoter: CMV
Tag / Fusion Protein: SBP (N terminal on insert)

Cloning Information
Cloning method: Restriction Enzyme
5′ cloning site: BamHI (not destroyed)
3′ cloning site: EcoRV (not destroyed)
5′ sequencing primer: CMV-F
Research Area Cell Cycle
Notes Vector backbone: pcDNA5/FRT/TO
Vector type: Mammalian Expression

Growth in Bacteria
Bacterial Resistance(s): Ampicillin
Growth Temperature: 37°C
Growth Strain(s): DH5alpha
Copy number: High Copy

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Inventor Information