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PEO1-CDDP Cell Line

Invented by Simon Langdon at The University of Edinburgh

Info

Catalogue Number 151784
Antigen/Gene or Protein Targets Human ovarian cancer; oestrogen receptor, drug resistance, cisplatin resistance
Parental Line PEO1
Host Human
Tissue Ovary
Disease Keywords Adenocarcinoma
Model Tumour line
Relevance The PEO1-CDDP Cell Line is an in-vitro model to understand the changes in EGF signalling after the onset of cisplatin resistance. Coupled with this change in drug sensitivity, this model also has a modified responsiveness to ligands of the erbB receptor family. This model will be helpful in studying the changes in cellular signalling after drug treatment, which are currently attracting a great deal of interest and this model will be of particular relevance when cytotoxic therapy is combined with signalling inhibitors.
Production Details The PE01CDDP cell line was established by in vitro exposure of the PE01 line to cisplatin commencing at 25 nmol/L cisplatin and increasing to 1 Amol/L over a period of 4 months. The resistant cell line thus obtained was then passaged in the absence of cisplatin for a further 4 months to ensure that the resistance remained stable. PEO1 CDDP is from the same patient as the PEO1, PEO4 and PEO6 cell lines (ECACC Catalogue numbers 10032308, 10032309 & 10032310).
Research Area Cancer, Cell Signaling & Signal Transduction, Drug Discovery & Development
Recommended Growing Conditions RPMI 1640 containing 10% heat-inactivated FCS, penicillin (100 units/mL), and streptomycin (100 ug/mL). Cells were maintained routinely at 37oC in a humidified atmosphere of 5% CO2 in air.
Notes Cisplatin Resistance
Cellosaurus ID CVCL_Y032

References: 1 entry

Macleod et al. 2005. Cancer Res. 65(15):6789-800. PMID: 16061661.


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References: 1 entry

Macleod et al. 2005. Cancer Res. 65(15):6789-800. PMID: 16061661.


Add a reference

References: 1 entry

Macleod et al. 2005. Cancer Res. 65(15):6789-800. PMID: 16061661.


Add a reference


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