anti-his tag iBody small molecule (tool compound)
Invented by Jan Konvalinka , Jiří Schimer , Tomas Knedlik , Vaclav Navratil , Jan Tykvart , Frantisek Sedlak , Pavel Majer , Petr Cigler , Vladimir Subr , Karel Ulbich , Jiri Strohalm , Sacha Pavel
Invented at Institute of Organic Chemistry and Biochemistry , Institute of Macromolecular Chemistry , Charles University
- Datasheet
- References (3)
- Inventor Info
Info
| Catalogue Number | 157996 |
| Antigen/Gene or Protein Targets | Targeting poly-histidine (HIS) tag |
| Synonyms | Polyhistidine; Poly-histidine; Hexahistidine; Hexa-histidine |
| Relevance |
A polyhistidine-tag is an amino acid motif in proteins that typically consists of at least six histidine (His) residues, often at the N- or C-terminus of the protein. - iBodies® are capable of replacing antibodies in biomedical applications such as ELISA, flow cytometry, confocal microscopy, immunocytochemistry, Western Blot and immunoprecipitation. - These iBodies® consist of an N-(2-hydroxypropyl)methacrylamide (HPMA) copolymer decorated with low-molecular-weight compounds that function as targeting ligand (e.g., enzyme inhibitors, receptor ligands), affinity anchor (e.g., biotin) and/or imaging probe (various fluorophores). - These iBodies® have been used for enzyme inhibition, protein isolation, immobilization, quantification and live cell imaging as well as for classical Western Blot application. The system is remarkably modular and can be used for different biological/biochemistry assays. |
| Key Attributes |
HPMA conjugate characteristics: MW: 98 kDa #Biotins/molecule: 13 Fluorochrome name: ATTO 488 #Fluorochrome/molecule: 10 |
| Research Area | Cell Signaling & Signal Transduction, Other |
| Notes | iBodies(R) is a registered trade mark of IOCB Tech s.r.o. |
Antigen/Gene or Protein Targets: Targeting neuraminidase using a neuramindase specific inhibitor
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Antigen/Gene or Protein Targets: Targeting human Carbonic Anhydrase (CAIX) using CAIX specific inhibitor
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Antigen/Gene or Protein Targets: Targeting HIV-1 protease using HIV1 PR specific inhibitor
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Antigen/Gene or Protein Targets: Tageting Glutathione S-Transferase (GST) tagged proteins using GST-specific inhibitor
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Antigen/Gene or Protein Targets: Targeting human Glutamate Carboxypeptidase II (GPCII) using GPCII specific inhibitor
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References: 3 entries
Šimon et al. 2018. ACS Chem Biol. 13(12):3333-3342. PMID: 30489064.
Dvořáková et al. 2017. J Med Chem. 60(20):8385-8393. PMID: 28953383.
Šácha et al. 2016. Angew Chem Int Ed Engl. 55(7):2356-60. PMID: 26749427.
Add a reference
References: 3 entries
Šimon et al. 2018. ACS Chem Biol. 13(12):3333-3342. PMID: 30489064.
Dvořáková et al. 2017. J Med Chem. 60(20):8385-8393. PMID: 28953383.
Šácha et al. 2016. Angew Chem Int Ed Engl. 55(7):2356-60. PMID: 26749427.
Add a reference
Inventor Information
Inventors
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Jan Konvalinka |
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Jiří Schimer |
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Tomas Knedlik |
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Vaclav Navratil |
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Jan Tykvart |
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Frantisek Sedlak |
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Pavel Majer |
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Petr Cigler |
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Vladimir Subr |
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Karel Ulbich |
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Jiri Strohalm |
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Sacha Pavel |
