Negative control iBody (ATTO488)
Invented by Jiri Strohalm , Karel Ulbich , Petr Cigler , Pavel Majer , Frantisek Sedlak , Tomas Knedlik , Jiri Schimer , Jan Konvalink , Pavel Sacha , Sub Vladlmir , Jan Tykvart , Vaclav Navratil
Invented at Institute of Organic Chemistry and Biochemistry , Charles University , Institute of Macromolecular Chemistry
- Datasheet
- References (3)
- Inventor Info
Info
| Catalogue Number | 157770 |
| Relevance |
- iBodies® are capable of replacing antibodies in biomedical applications such as ELISA, flow cytometry, confocal microscopy, immunocytochemistry, Western Blot and immunoprecipitation. - These iBodies® consist of an N-(2-hydroxypropyl)methacrylamide (HPMA) copolymer decorated with low-molecular-weight compounds that function as targeting ligand (e.g., enzyme inhibitors, receptor ligands), affinity anchor (e.g., biotin) and/or imaging probe (various fluorophores). - These iBodies® have been used for enzyme inhibition, protein isolation, immobilization, quantification and live cell imaging as well as for classical Western Blot application. The system is remarkably modular and can be used for different biological/biochemistry assays. |
| Research Area | Cancer, Cell Signaling & Signal Transduction, Other |
| Storage | Storage at -20°C; solubility H2O; stable at RT, 4°C and -80°C |
| Notes |
HPMA conjugate characteristics: MW: 78 041 g/mol #Biotins/molecule: 8 Fluorochrome name: ATTO488 #Fluorochrome/molecule: 5 |
Antigen/Gene or Protein Targets: Tageting Glutathione S-Transferase (GST) tagged proteins using GST-specific inhibitor
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reviews
3
references
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supplier
Antigen/Gene or Protein Targets: Targeting poly-histidine (HIS) tag
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reviews
3
references
1
supplier
Antigen/Gene or Protein Targets: Targeting HIV-1 protease using HIV1 PR specific inhibitor
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reviews
3
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1
supplier
Antigen/Gene or Protein Targets: Targeting human Glutamate Carboxypeptidase II (GPCII) using GPCII specific inhibitor
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reviews
3
references
1
supplier
Antigen/Gene or Protein Targets: Targeting human Carbonic Anhydrase (CAIX) using CAIX specific inhibitor
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reviews
3
references
1
supplier
Antigen/Gene or Protein Targets: Targeting neuraminidase using a neuramindase specific inhibitor
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reviews
3
references
1
supplier
References: 3 entries
Šimon et al. 2018. ACS Chem Biol. 13(12):3333-3342. PMID: 30489064.
Dvořáková et al. 2017. J Med Chem. 60(20):8385-8393. PMID: 28953383.
Šácha et al. 2016. Angew Chem Int Ed Engl. 55(7):2356-60. PMID: 26749427.
Add a reference
References: 3 entries
Šimon et al. 2018. ACS Chem Biol. 13(12):3333-3342. PMID: 30489064.
Dvořáková et al. 2017. J Med Chem. 60(20):8385-8393. PMID: 28953383.
Šácha et al. 2016. Angew Chem Int Ed Engl. 55(7):2356-60. PMID: 26749427.
Add a reference
Inventor Information
Inventors
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Jiri Strohalm |
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Karel Ulbich |
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Petr Cigler |
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Pavel Majer |
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Frantisek Sedlak |
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Tomas Knedlik |
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Jiri Schimer |
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Jan Konvalink |
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Pavel Sacha |
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Sub Vladlmir |
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Jan Tykvart |
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Vaclav Navratil |
