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Catalogue Number 154832
Applications N/A
Antigen/Gene or Protein Targets Rad51
Reactivity Bovine, Human and Pig
Relevance Recombinant antibody which binds RAD51 recombinase protein, associated with DNA repair pathway.

Background and Research Application
RAD51 is a eukaryotic homologue of E. coli RecA, a recombinase, and a component of the homologous recombination DNA repair pathway. RAD51 forms a nucleoprotein filament (through binding RAD52 and single stranded DNA that are exposed following double strand breaks) that initiates recombination. RAD51 catalyses the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. Mutations in the gene can results in breast cancer, mirror movements 2 and Fanconi anaemia.
This is a recombinant version of the anti-RAD51 monoclonal antibody.
Host Mouse
Immunogen Full length recombinant human RAD51 protein
Subclass IgG1
Research Area DNA Damage and Repair, Epigenetics & Nuclear Signalling
Immunogen UniProt ID Q06609
Notes Production Details
Purified using multi-step affinity chromatography with protein A.

Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.

Points of Interest
Anti-RAD51 antibody has been used to immunohistochemically stain biopsy and resected specimens of oesophagus and squamous cell carcinoma of the oesophagus.

Concentration
1mg/ml as standard

References

There are 6 reference entries for this reagent.

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References: 6 entries

Barlow et al. 1997. EMBO J. 16(17):5207-15. PMID: 9311981.

Distribution of the Rad51 recombinase in human and mouse spermatocytes.

Endopolyploid cells produced after severe genotoxic damage have the potential to repair DNA double strand breaks.

Ivanov et al. 2003. J Cell Sci. 116(Pt 20):4095-106. PMID: 12953071.

Masson et al. 1999. EMBO J. 18(22):6552-60. PMID: 10562567.

The meiosis-specific recombinase hDmc1 forms ring structures and interacts with hRad51.


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References: 6 entries

Barlow et al. 1997. EMBO J. 16(17):5207-15. PMID: 9311981.

Distribution of the Rad51 recombinase in human and mouse spermatocytes.

Endopolyploid cells produced after severe genotoxic damage have the potential to repair DNA double strand breaks.

Ivanov et al. 2003. J Cell Sci. 116(Pt 20):4095-106. PMID: 12953071.

Masson et al. 1999. EMBO J. 18(22):6552-60. PMID: 10562567.

The meiosis-specific recombinase hDmc1 forms ring structures and interacts with hRad51.


Add a reference