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Monoclonal Antibodies

Anti-DAF [67]

Invented by Prof Nancy Hogg from Cancer Research UK London Research Institute: Lincoln's Inn Fields
Invented at Cancer Research UK London Research Institute: Lincoln's Inn Fields
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Adapted from Gheorghe et al. 2011. PLoS One. 6(1):e16378. PMID: 21298002. MPGES1 expression in synovial lining fibroblasts before and 16 weeks after initiation of rituximab therapy. Double immunofluorescence pictures show the presence of MPGES1 (red) expression in CD55 positive fibroblasts (green) in the rheumatoid tissue before rituximab initiation (A) and 16 weeks later (B). Original magnification 500x. Arrows point to double stained cells.
Adapted from Leitinger B, Hogg N. The involvement of lipid rafts in the regulation of integrin function. J Cell Sci. 2002 Mar 1;115(Pt 5):963-72. PMID: 11870215. Integrin LFA-1 has higher affinity for lipid raft patches on J-β2.7 cells expressing ΔI-LFA-1 than on J-β2.7 cells expressing wt LFA-1. Cells were incubated with TRITC-conjugated Ctx-B, then crosslinked with rabbit anti-Ctx-B antibody. Cells were fixed and stained with mAbs against LFA-1 (A), DAF (B), or TfR (C), followed by Alexa 488-conjugated goat anti-mouse IgG. Single optical sections taken at mid-height of the cells are shown. Top panels, ΔI-LFA-1-expressing cells; bottom panels, wt LFA-1-expressing cells. Cell surface proteins, green; Ctx-B, red. Data are representative of six experiments (A), and three experiments (B,C). Bars, 10 μm.
Adapted from Kamada K, Arita N, Tsubaki T, Takubo N, Fujino T, Soga Y, Miyazaki T, Yamamoto H, Nose M. Expression of sphingosine kinase 2 in synovial fibroblasts of rheumatoid arthritis contributing to apoptosis by a sphingosine analogue, FTY720. Pathol Int. 2009 Jun;59(6):382-9. doi: 10.1111/j.1440-1827.2009.02381.x. PMID: 19490468. Sphingosine kinase 2 (SPHK2)-positive cells in the synovial tissues of arthritis patients. (a–h) Frozen sections of the synovium obtained from the early rheumatoid arthritis (RA) patients showing (a) the proliferative lesion with synovial lining cells and synovial fibroblasts in the sub-lining region and (e) the lesion with angioneogenesis in another case (HE). (b–d,f–h) Double staining with anti-SPHK2 (red) and anti-CD68 (green). (c) SPHK2 is highly expressed in synovial fibroblasts along the sub-lining layer. (b) Positive cells for SPHK2 and CD68 are different to each other as shown by merging. (g) SPHK2 is also positive in the perivascular fibroblastic cells, but (f) almost all of them are weakly positive for CD68 as shown by merging. (i–l) Immunofluorescence of frozen sections of synovium obtained from (i,k) a patient with early-stage rheumatoid arthritis and (j,l) an osteoarthritis patient, on confocal laser microscopy. (i,j) Double staining with anti-SPHK2 (red) and anti-CD68 (green) more clearly demonstrates that SPHK2-positive cells localized in the sub-lining regions (single arrow) and the cells positive for CD68 in the lining layer with weakly positive for SPHK2 (double arrows). (j) Perivascular cells are positive for only SPHK2 (arrowhead). (k,l) Double staining with anti-SPHK2 (red) and anti-CD55 (green) shows that the CD55-positive cells are localized in the sub-lining regions, some of them are strongly positive for SPHK2 (single arrow). (k) Perivascular cells are negative for CD55 (arrowhead). Bars, 100 mm.
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Catalogue Number 151060
Applications FACS IHC IF WB
Antigen/Gene or Protein Targets Decay accelerating factor (DAF, CD55)
Synonyms CD55
Reactivity Human
Relevance Decay Accelerating Factor is a distinctive marker of synovium. Decay Accelerating Factor protects tissues from attack by autologous complement by interfering with the assembly of C3 convertase.
Host Mouse
Immunogen Fibronectin purified human monocytes .
Subclass IgG1
Myeloma Used Sp2/0-Ag14
Recommended Growing Conditions DMEM + 5% FCS
Research Area Cell Type or Organelle Marker, Immunology

References

There are 7 reference entries for this reagent.

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References: 7 entries

Gheorghe et al. 2011. PLoS One. 6(1):e16378. PMID: 21298002.

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Prostaglandin E2 synthesizing enzymes in rheumatoid arthritis B cells and the effects of B cell depleting therapy on enzyme expression.

Europe PMC ID: 21298002

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Kamada et al. 2009. Pathol Int. 59(6):382-9. PMID: 19490468.

View  

Leitinger et al. 2002. J Cell Sci. 115(Pt 5):963-72. PMID: 11870215.

View  

The involvement of lipid rafts in the regulation of integrin function.

Europe PMC ID: 11870215

View  

Palmer et al. 1985. Clin Exp Immunol. 59(3):529-38. PMID: 3886218.

View  

Features of synovial membrane identified with monoclonal antibodies.

Europe PMC ID: 3886218

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References: 7 entries

Gheorghe et al. 2011. PLoS One. 6(1):e16378. PMID: 21298002.

View  

Prostaglandin E2 synthesizing enzymes in rheumatoid arthritis B cells and the effects of B cell depleting therapy on enzyme expression.

View  

Kamada et al. 2009. Pathol Int. 59(6):382-9. PMID: 19490468.

View  

Leitinger et al. 2002. J Cell Sci. 115(Pt 5):963-72. PMID: 11870215.

View  

The involvement of lipid rafts in the regulation of integrin function.

View  

Palmer et al. 1985. Clin Exp Immunol. 59(3):529-38. PMID: 3886218.

View  

Features of synovial membrane identified with monoclonal antibodies.

View  

Add a reference

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