Invented by Prof Emanuele Buratti from International Centre For Genetic Engineering And Biotechnology (ICGEB)
- References (2)
- Inventor Info
|Backbone Size (bp)
|This vector is a typical hybrid minigene that contains a ‘‘simplified’’ version of the gene that will be evaluated for pre-mRNA splicing. In particular, it contains at the 5' end an alfa-globin gene promoter and SV40 enhancer sequences to allow polymerase II transcription in the transfected cell lines. This is followed by a series of exonic and intronic sequences that may derive from a reporter gene or from the gene context itself. In this case, the reporter gene is composed of a-globin and fibronectin exons, while at the 3' end a functionally competent polyadenylation site, derived from the alfa-globin gene, is present. The genomic DNA region of interest that contains a putative splicing mutation is introduced into the minigene in a unique restriction site (NdeI).
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