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THUECa Cell Line

Invented by Prof Michael Apicella at The University Of Iowa

Info

Catalogue Number 153754
Antigen/Gene or Protein Targets Transformed Human Urethra Epithelial Cell line; Sexually transmitted diseases (STIs)
Host Human
Disease Keywords Neisseria gonorrhoeae infection, Gonorrhea
Model Immortalised Line
Relevance This transformed human urethera epithelial cell line was developed to study the invasion of Neisseria gonorrhoeae in urethral epithelial cells during gonococcal infection in men.

This cell line is phenotypically similiar and responds to gonococcal infection similar to primary cells based on immunofluorescence microscopy data. Cells stain positive for cytokeratin demonstrating immortalized cells are keratinocytes. Human asialoglycoprotein receptor expression and, IL-6 and IL-8 levels are increased after gonococcal infection similar to primary cells. This cell line is CD14 negative.
Production Details This cell line was derived from primary urethral epithelial cells (obtained from 15 male patients) immortalized by transduction with a retroviral vector expressing human papillomavirus E6E7 genes.
Conditional No
Research Area Immunology
Recommended Growing Conditions Prostate Epithelial Cell Growth Medium (PrEGM). Cells grow best on collagen-free lab plastic.

References

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References: 1 entry

Harvey et al. 2002. Infect Immun. 70(10):5808-15. PMID: 12228311.


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References: 1 entry

Harvey et al. 2002. Infect Immun. 70(10):5808-15. PMID: 12228311.


Add a reference