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HRA-19a1.1 Alpha2 B Cell Line

Info

Antigen/Gene or Protein Targets Wild-type alpha2 integrin
Parental Line HRA-19
Host Human
Disease Keywords Human colorectal adenocarcinoma
Relevance The HRA-19a1.1 Alpha2B cell line is a genetically modified clone of HRA-19a1.1 cells expressing expressing wild-type alpha2 integrin. Integrins are transmembrane receptors that are the bridges for cell-cell and cell-extracellular matrix (ECM) interactions.
HRA-19 has stem cell properties and undergoes a multilineage differentiation in serum free medium.
Production Details Genetically modified HRA-19a1.1 cells expressing wild-type alpha2 integrin
Research Area Cancer, Cell Structure and Motility, Drug Discovery & Development, Stem Cell Biology
Growth/Phenotype Keywords Morphology: Epithelial Growth Mode: Adherent
Recommended Growing Conditions Subculture Routine: Split sub-confluent cultures (70-80%) 1:3 to 1:4 weekly using 0.0125% trypsin/EDTA; 7.5% CO2; 37°C. Suggested seeding density 2-4x10,000 cells/cm².Culture Medium: DMEM (High glucose) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + G418 200µg/ml
Notes DNA Profile: STR-PCR Data:
Amelogenin: X,Y
CSF1PO: 12
D13S317: 11,12
D16S539: 11
D5S818: 11
D7S820: 9,12
THO1: 8
TPOX: 11
vWA: 16
Genetically Modified Organism Class 1 (GMO1)
Cellosaurus ID CVCL_2512

References: 10 entries

Henderson et al. 1996. Differentiation. 60(4):259-68. PMID: 8765056.

Kirkland et al. 1986. Br J Cancer. 53(6):779-85. PMID: 3718830.

Kirkland et al. 1986. Differentiation. 33(2):148-55. PMID: 3569697.

Kirkland et al. 1988. Cancer. 61(7):1359-63. PMID: 2449944.

Kirkland et al. 2008. J Biol Chem. 283(41):27612-9. PMID: 18664572.

Alpha2beta1 integrin regulates lineage commitment in multipotent human colorectal cancer cells.

Europe PMC ID: 18664572

Multilineage differentiation of cloned HRA-19 cells in serum-free medium: a model of human colorectal epithelial differentiation.

Europe PMC ID: 8765056

Clonal origin of columnar, mucous, and endocrine cell lineages in human colorectal epithelium.

Europe PMC ID: 2449944

Establishment and characterisation of six human colorectal adenocarcinoma cell lines.

Europe PMC ID: 3718830

Endocrine differentiation by a human rectal adenocarcinoma cell line (HRA-19).

Europe PMC ID: 3569697


Add a reference

References: 10 entries

Henderson et al. 1996. Differentiation. 60(4):259-68. PMID: 8765056.

Kirkland et al. 1986. Br J Cancer. 53(6):779-85. PMID: 3718830.

Kirkland et al. 1986. Differentiation. 33(2):148-55. PMID: 3569697.

Kirkland et al. 1988. Cancer. 61(7):1359-63. PMID: 2449944.

Kirkland et al. 2008. J Biol Chem. 283(41):27612-9. PMID: 18664572.

Alpha2beta1 integrin regulates lineage commitment in multipotent human colorectal cancer cells.

Europe PMC ID: 18664572

Multilineage differentiation of cloned HRA-19 cells in serum-free medium: a model of human colorectal epithelial differentiation.

Europe PMC ID: 8765056

Clonal origin of columnar, mucous, and endocrine cell lineages in human colorectal epithelium.

Europe PMC ID: 2449944

Establishment and characterisation of six human colorectal adenocarcinoma cell lines.

Europe PMC ID: 3718830

Endocrine differentiation by a human rectal adenocarcinoma cell line (HRA-19).

Europe PMC ID: 3569697


Add a reference

References: 10 entries

Henderson et al. 1996. Differentiation. 60(4):259-68. PMID: 8765056.

Kirkland et al. 1986. Br J Cancer. 53(6):779-85. PMID: 3718830.

Kirkland et al. 1986. Differentiation. 33(2):148-55. PMID: 3569697.

Kirkland et al. 1988. Cancer. 61(7):1359-63. PMID: 2449944.

Kirkland et al. 2008. J Biol Chem. 283(41):27612-9. PMID: 18664572.

Alpha2beta1 integrin regulates lineage commitment in multipotent human colorectal cancer cells.

Multilineage differentiation of cloned HRA-19 cells in serum-free medium: a model of human colorectal epithelial differentiation.

Clonal origin of columnar, mucous, and endocrine cell lineages in human colorectal epithelium.

Establishment and characterisation of six human colorectal adenocarcinoma cell lines.

Endocrine differentiation by a human rectal adenocarcinoma cell line (HRA-19).


Add a reference


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