Anti-IGF [1R 24-55]
Invented by Kenneth Siddle at University of Cambridge
- Datasheet
- References (3)
- Inventor Info
Info
| Applications | FACS IP |
| Antigen/Gene or Protein Targets | Human insulin-like growth factor 1 receptor |
| Reactivity | Human |
| Relevance | Insulin-like growth factor I (IGF-I) is a 70 aa polypeptide hormone with endocrine, paracrine, and autocrine effects. It shares >60% homology with IGF-II and by 50% homology with proinsulin structures. This clone has been shown to cause 35-60% inhibition of IGF binding and has been used for immunoprecipitation and blocking assays. |
| Host | Mouse |
| Immunogen | Antibody directed at type-1 insulin-like growth factor receptor (440-586 epitope) |
| Subclass | IgG1 |
| Notes | Antibodies 17-69 and 24-55 were isolated in separate fusions using spleen cells from different mice and therefore it is very possible that they have different epitopes. Reaction of both antibodies within the same rather large region (AA440-586 - effectively the first FnIII domain) was demonstrated using available IR/IGFR domain swap chimeras, but that does not necessarily mean they share a common epitope. Indeed, 17-69 and 24-55 can bind simultaneously to IGF, and they exhibit significant differences in properties (for instance 17-69 reacts very well with IR/IGFR hybrids, but 24-55 reacts very poorly with hybrids). Both antibodies moderately inhibit IGF-1 binding. Neither is "better" - they are just different. |
| Research Area | Cancer, Cell Signaling & Signal Transduction, Developmental Biology |
References: 3 entries
Schumacher et al. 1993. J Biol Chem. 268(2):1087-94. PMID: 7678247.
Soos et al. 1992. J Biol Chem. 267(18):12955-63. PMID: 1377676.
A panel of monoclonal antibodies for the type I insulin-like growth factor receptor. Epitope mapping, effects on ligand binding, and biological activity.
Europe PMC ID: 1377676
Add a reference
References: 3 entries
Schumacher et al. 1993. J Biol Chem. 268(2):1087-94. PMID: 7678247.
Soos et al. 1992. J Biol Chem. 267(18):12955-63. PMID: 1377676.
A panel of monoclonal antibodies for the type I insulin-like growth factor receptor. Epitope mapping, effects on ligand binding, and biological activity.
Add a reference
Inventor Information
Inventors
|
Kenneth Siddle |
