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FNB6 hTERT Cell Line

Info

Catalogue Number 151634
Antigen/Gene or Protein Targets FNB6 cell line immortalized by ectopic expression of hTERT
Host Human
Tissue Oral Cavity
Disease Keywords Oral Dysplasias
Model Immortalised Line
Relevance The FNB6 hTERT Cell Line is a primary human atypical mortal dysplasia oral cell line, which has been immortalised by ectopic expression of hTERT. Acquisition of immortality at the dysplasia stage or oral cancer progression is consistently associated with a number of changes; namely loss of retinoic acid receptor (RAR)-beta and p16 INK4a expression, p53 mutations and activation of telomerase.
Production Details Normal FNB6 was obtained from a healthy volunteer; this culture senesced at 11.5 PDs, respectively. Biopsies were trypsinized and cultured until a growing population of cells was obtained in a 9-cm plate and then passaged once to give a stock culture that was frozen. All cells were maintained on irradiated 3T3 feeders in FAD+ medium.
Research Area Cancer, Cell Cycle, Drug Discovery & Development
Growth/Phenotype Keywords Epithelial
Recommended Growing Conditions Cells were maintained on irradiated swiss 3T3 feeders in FAD+ medium (1:3 Ham's F-l2/DMEM) with 10% FCS, insulin5ug/ml, EGF10ng/ml, transferrin5ug/ml, cholera toxin10ng/ml, hydrocortisone 400ng/ml, and adenine30ug/ml(use fresh)
Cellosaurus ID CVCL_F734

References: 4 entries

Hakami et al. 2014. Br J Cancer. 111(4):807-16. PMID: 25010866.

The roles of HOXD10 in the development and progression of head and neck squamous cell carcinoma (HNSCC).

Europe PMC ID: 25010866

McGregor et al. 2002. Cancer Res. 62(16):4757-66. PMID: 12183435.

Molecular changes associated with oral dysplasia progression and acquisition of immortality: potential for its reversal by 5-azacytidine.

Europe PMC ID: 12183435


Add a reference

References: 4 entries

Hakami et al. 2014. Br J Cancer. 111(4):807-16. PMID: 25010866.

The roles of HOXD10 in the development and progression of head and neck squamous cell carcinoma (HNSCC).

Europe PMC ID: 25010866

McGregor et al. 2002. Cancer Res. 62(16):4757-66. PMID: 12183435.

Molecular changes associated with oral dysplasia progression and acquisition of immortality: potential for its reversal by 5-azacytidine.

Europe PMC ID: 12183435


Add a reference

References: 4 entries

Hakami et al. 2014. Br J Cancer. 111(4):807-16. PMID: 25010866.

The roles of HOXD10 in the development and progression of head and neck squamous cell carcinoma (HNSCC).

McGregor et al. 2002. Cancer Res. 62(16):4757-66. PMID: 12183435.

Molecular changes associated with oral dysplasia progression and acquisition of immortality: potential for its reversal by 5-azacytidine.


Add a reference


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