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|Applications||ELISA FACS IF IP WB|
|Antigen/Gene or Protein Targets||BZLF1|
|Relevance||The Epstein-Barr Virus (EBV), also called Human herpesvirus 4 (HHV-4), is a virus of the herpes family and is one of the most common viruses in humans. BZLF1 functions as a transcriptional activator of EBV early gene expression and is essential for the viral transition between latent and lytic replication. In addition to its role in the EBV life cycle, BZLF1 also has profound effects upon the host cellular environment, including disruption of cell cycle regulation, signal transduction pathways, and transcription.|
|Immunogen||Full length recombinant EBV antigen ZEBRA (BZLF1)|
|Positive Control||B95.8 & P3HR-1 cell lines (induced with phorbol ester to give greater percentage positive) or Akata-BL induced with anti-IgG.|
|Recommended Growing Conditions||RPMI 1640/10% FCS/1mM pyruvate/Pen&Strep (supplement with 10% BM Condimed HI (Roche Diagnostics) if the cells are poor or slwo to grow.)|
|Notes||Reactivity: Epstein-Barr Virus (EBV)|
|Research Area||Cell Cycle, Cell Signaling & Signal Transduction, Epigenetics & Nuclear Signalling, Virology|
References: 5 entries
Robinson et al. 2011. J Virol. 85(17):8940-53. PMID: 21697476.
Bristol et al. 2010. J Virol. 84(23):12362-74. PMID: 20861254.
Inman et al. 2001. J Virol. 75(5):2400-10. PMID: 11160743.
Young et al. 1991. J Virol. 65(6):2868-74. PMID: 1851858.
Rowe et al. 1991. J Exp Med. 173(1):147-58. PMID: 1845872.
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