Anti-BZLF1 [BZ.1]
Invented by Prof Martin Rowe at University Of Birmingham
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Info
| Catalogue Number | 151473 |
| Applications | ELISA FACS IF IP WB |
| Antigen/Gene or Protein Targets | BZLF1 |
| Reactivity | Virus |
| Relevance | The Epstein-Barr Virus (EBV), also called Human herpesvirus 4 (HHV-4), is a virus of the herpes family and is one of the most common viruses in humans. BZLF1 functions as a transcriptional activator of EBV early gene expression and is essential for the viral transition between latent and lytic replication. In addition to its role in the EBV life cycle, BZLF1 also has profound effects upon the host cellular environment, including disruption of cell cycle regulation, signal transduction pathways, and transcription. |
| Host | Mouse |
| Immunogen | Full length recombinant EBV antigen ZEBRA (BZLF1) |
| Subclass | IgG1 |
| Positive Control | B95.8 & P3HR-1 cell lines (induced with phorbol ester to give greater percentage positive) or Akata-BL induced with anti-IgG. |
| Myeloma Used | P3X63Ag8.653 |
| Recommended Growing Conditions | RPMI 1640/10% FCS/1mM pyruvate/Pen&Strep (supplement with 10% BM Condimed HI (Roche Diagnostics) if the cells are poor or slwo to grow.) |
| Strain | Balb/c |
| Notes | Reactivity: Epstein-Barr Virus (EBV) |
| Research Area | Cell Cycle, Cell Signaling & Signal Transduction, Epigenetics & Nuclear Signalling, Virology |
References: 10 entries
Robinson et al. 2011. J Virol. 85(17):8940-53. PMID: 21697476.
Cellular transcription factor Oct-1 interacts with the Epstein-Barr virus BRLF1 protein to promote disruption of viral latency.
Europe PMC ID: 21697476
Bristol et al. 2010. J Virol. 84(23):12362-74. PMID: 20861254.
The Epstein-Barr virus BZLF1 protein inhibits tumor necrosis factor receptor 1 expression through effects on cellular C/EBP proteins.
Europe PMC ID: 20861254
Inman et al. 2001. J Virol. 75(5):2400-10. PMID: 11160743.
FACS IF
Activators of the Epstein-Barr virus lytic program concomitantly induce apoptosis, but lytic gene expression protects from cell death.
Europe PMC ID: 11160743
Young et al. 1991. J Virol. 65(6):2868-74. PMID: 1851858.
Differentiation-associated expression of the Epstein-Barr virus BZLF1 transactivator protein in oral hairy leukoplakia.
Europe PMC ID: 1851858
Epstein-Barr virus (EBV)-associated lymphoproliferative disease in the SCID mouse model: implications for the pathogenesis of EBV-positive lymphomas in man.
Europe PMC ID: 1845872
Rowe et al. 1991. J Exp Med. 173(1):147-58. PMID: 1845872.
IF
Add a reference
References: 10 entries
Robinson et al. 2011. J Virol. 85(17):8940-53. PMID: 21697476.
Cellular transcription factor Oct-1 interacts with the Epstein-Barr virus BRLF1 protein to promote disruption of viral latency.
Europe PMC ID: 21697476
Bristol et al. 2010. J Virol. 84(23):12362-74. PMID: 20861254.
The Epstein-Barr virus BZLF1 protein inhibits tumor necrosis factor receptor 1 expression through effects on cellular C/EBP proteins.
Europe PMC ID: 20861254
Inman et al. 2001. J Virol. 75(5):2400-10. PMID: 11160743.
FACS IF
Activators of the Epstein-Barr virus lytic program concomitantly induce apoptosis, but lytic gene expression protects from cell death.
Europe PMC ID: 11160743
Young et al. 1991. J Virol. 65(6):2868-74. PMID: 1851858.
Differentiation-associated expression of the Epstein-Barr virus BZLF1 transactivator protein in oral hairy leukoplakia.
Europe PMC ID: 1851858
Epstein-Barr virus (EBV)-associated lymphoproliferative disease in the SCID mouse model: implications for the pathogenesis of EBV-positive lymphomas in man.
Europe PMC ID: 1845872
Rowe et al. 1991. J Exp Med. 173(1):147-58. PMID: 1845872.
IF
Add a reference
References: 10 entries
Robinson et al. 2011. J Virol. 85(17):8940-53. PMID: 21697476.
Cellular transcription factor Oct-1 interacts with the Epstein-Barr virus BRLF1 protein to promote disruption of viral latency.
Bristol et al. 2010. J Virol. 84(23):12362-74. PMID: 20861254.
The Epstein-Barr virus BZLF1 protein inhibits tumor necrosis factor receptor 1 expression through effects on cellular C/EBP proteins.
Inman et al. 2001. J Virol. 75(5):2400-10. PMID: 11160743.
FACS IF
Activators of the Epstein-Barr virus lytic program concomitantly induce apoptosis, but lytic gene expression protects from cell death.
Young et al. 1991. J Virol. 65(6):2868-74. PMID: 1851858.
Differentiation-associated expression of the Epstein-Barr virus BZLF1 transactivator protein in oral hairy leukoplakia.
Epstein-Barr virus (EBV)-associated lymphoproliferative disease in the SCID mouse model: implications for the pathogenesis of EBV-positive lymphomas in man.
Rowe et al. 1991. J Exp Med. 173(1):147-58. PMID: 1845872.
IF
Add a reference
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