Cancer Research Technology
Log in Register
Menu

HeLa Flp-In SLC7A5
NEW

Invented by Matthias Hentze
Invented at European Molecular Biology Laboratory

Info

Catalogue Number 160839
Antigen/Gene or Protein Targets YBX3
Parental Line HeLa FLP-IN T-REx
Model Knock-Out
Relevance To identify genes whose expression is controlled by YBX3
Production Details Adherent HeLa cells were grown at 37°C and 5% CO2 in DMEM, supplemented with 10% FBS Gold (PAA), 1% Penicillin/Streptomycin (Sigma) and 1% L-glutamine (GIBCO)
Research Area Metabolism
Recommended Growing Conditions Grown in medium containing blasticidine (5mg/ml) and hygromycin (200mg/ml).
Notes Cells were grown until 70%–80% confluent, trypsinized, counted (Biorad TC20) and 200,000 cells were seeded per well in a 6-well dish for reverse transfection of siRNAs following the manufacturer’s recommendations (Lipofectamine RNAiMax, Invitrogen, cat# 137780755). YBX3 and control siRNAs were obtained from Dharmacon (YBX3 pool: SMARTpool ON-Targetplus L-015793-00 or YBX3 3′ UTR targeting: ON-Targetplus L-015793-07; and control pool: ON-Targetplus non-targeting siRNAs 1-4). The siRNA used to target the YBX3s 3′ UTR was YBX3 smartpool J-015793-07. Cells were harvested 48 hours after transfections.

References

There are 1 reference entries for this reagent.

View All References

References: 1 entry

The RNA-Binding Protein YBX3 Controls Amino Acid Levels by Regulating SLC mRNA Abundance


Add a reference

References: 1 entry

The RNA-Binding Protein YBX3 Controls Amino Acid Levels by Regulating SLC mRNA Abundance


Add a reference

Inventor Information