Anti-Keratin6 [LHK6]
Invented at Queen Mary University of London
- Datasheet
- References (8)
- Inventor Info
Info
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| Catalogue Number | 151440 |
| Applications | ELISA FACS IHC IF WB |
| Antigen/Gene or Protein Targets | Keratin 6 |
| Synonyms |
CK6A, CK6B, CK6C, CK6D, CK6E, Keratin Type II Cytoskeletal 6A, Keratin Type II Cytoskeletal 6B, Keratin Type II Cytoskeletal 6C, Keratin Type II Cytoskeletal 6D, Keratin Type II Cytoskeletal 6E, KRT6, KRT6A, KRT6B, KRT6C, KRT6D, KRT6E Human Entrez Gene ID 3853-KRT6A, 3854-KRT6B, 286887-KRT6C Human SwissProt P02538-KRT6A, P04259-KRT6B, P48668-KRT6C Human Unigene 700779 Human Gene Symbol KRT6A; KRT6B; KRT6C Human Chromosome Location 12q13.13; 12q13.13; 12q13.13 |
| Reactivity | Human and Mouse |
| Relevance | Clone LHK6 recognizes a protein of 56kDa, identified as cytokeratin 6 (CK6). In humans, multiple isoforms of Cytokeratin 6 (6A-6F), encoded by several highly homologous genes, have distinct tissue expression patterns. Keratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I keratin (keratin 9 to 23) and on type II kerating (1 to 8). Keratins demonstrate tissue and differentiation specific expression profiles. Keratins 6,16 and 17 are expressed in suprabasasl keratinocytes of wounded epidermis. Keratin 6 is found in acrosyringeal keratinocyes in normal skin. LHK6 is a very good marker for hyperproliferative keratinocytes. |
| Host | Mouse |
| Immunogen | 11 amino aid sequence GSSTIKYTTTS of K6b C terminal |
| Positive Control | Tonsil or basal cell carcinoma |
| Subclass | IgG2a kappa |
| Molecular Weight (kDa) | 55-57 |
| Myeloma Used | Sp2/0-Ag14 |
| Recommended Growing Conditions | DMEM + 5% FCS |
| Notes |
Cellular Localization: Cytoplasmic Suggested Dilutions: Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (0.5-1ug/ml); Immunohistology (Formalin-fixed) (0.1-0.2ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes) |
| Research Area | Cell Signaling & Signal Transduction |
References: 8 entries
Toivola et al. 2002. Mol Biol Cell. 13(6):1857-70. PMID: 12058054.
Type II keratins are phosphorylated on a unique motif during stress and mitosis in tissues and cultured cells.
Europe PMC ID: 12058054
Porter et al. 2000. Lab Invest. 80(11):1701-10. PMID: 11092530.
K15 expression implies lateral differentiation within stratified epithelial basal cells.
Europe PMC ID: 11092530
Machesney et al. 1998. Am J Pathol. 152(5):1133-41. PMID: 9588880.
Activated keratinocytes in the epidermis of hypertrophic scars.
Europe PMC ID: 9588880
Wetzels et al. 1991. Am J Pathol. 138(3):751-63. PMID: 1705754.
Basal cell-specific and hyperproliferation-related keratins in human breast cancer.
Europe PMC ID: 1705754
Add a reference
References: 8 entries
Toivola et al. 2002. Mol Biol Cell. 13(6):1857-70. PMID: 12058054.
Type II keratins are phosphorylated on a unique motif during stress and mitosis in tissues and cultured cells.
Porter et al. 2000. Lab Invest. 80(11):1701-10. PMID: 11092530.
K15 expression implies lateral differentiation within stratified epithelial basal cells.
Machesney et al. 1998. Am J Pathol. 152(5):1133-41. PMID: 9588880.
Activated keratinocytes in the epidermis of hypertrophic scars.
Wetzels et al. 1991. Am J Pathol. 138(3):751-63. PMID: 1705754.
Basal cell-specific and hyperproliferation-related keratins in human breast cancer.
Add a reference
