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Anti-Keratin6 [LHK6]


Catalogue Number 151440
Antigen/Gene or Protein Targets Keratin 6
Synonyms CK6A, CK6B, CK6C, CK6D, CK6E, Keratin Type II Cytoskeletal 6A, Keratin Type II Cytoskeletal 6B, Keratin Type II Cytoskeletal 6C, Keratin Type II Cytoskeletal 6D, Keratin Type II Cytoskeletal 6E, KRT6, KRT6A, KRT6B, KRT6C, KRT6D, KRT6E
Human Entrez Gene ID 3853-KRT6A, 3854-KRT6B, 286887-KRT6C
Human SwissProt P02538-KRT6A, P04259-KRT6B, P48668-KRT6C
Human Unigene 700779
Human Gene Symbol KRT6A; KRT6B; KRT6C
Human Chromosome Location 12q13.13; 12q13.13; 12q13.13
Reactivity Human and Mouse
Relevance Clone LHK6 recognizes a protein of 56kDa, identified as cytokeratin 6 (CK6). In humans, multiple isoforms of Cytokeratin 6 (6A-6F), encoded by several highly homologous genes, have distinct tissue expression patterns. Keratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I keratin (keratin 9 to 23) and on type II kerating (1 to 8). Keratins demonstrate tissue and differentiation specific expression profiles. Keratins 6,16 and 17 are expressed in suprabasasl keratinocytes of wounded epidermis. Keratin 6 is found in acrosyringeal keratinocyes in normal skin. LHK6 is a very good marker for hyperproliferative keratinocytes.
Host Mouse
Immunogen 11 amino aid sequence GSSTIKYTTTS of K6b C terminal
Subclass IgG2a kappa
Positive Control Tonsil or basal cell carcinoma
Molecular Weight (kDa) 55-57
Myeloma Used Sp2/0-Ag14
Recommended Growing Conditions DMEM + 5% FCS
Notes Cellular Localization: Cytoplasmic
Suggested Dilutions: Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (0.5-1ug/ml); Immunohistology (Formalin-fixed) (0.1-0.2ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Research Area Cell Signaling & Signal Transduction

References: 4 entries

Toivola et al. 2002. Mol Biol Cell. 13(6):1857-70. PMID: 12058054.

Porter et al. 2000. Lab Invest. 80(11):1701-10. PMID: 11092530.

Machesney et al. 1998. Am J Pathol. 152(5):1133-41. PMID: 9588880.

Wetzels et al. 1991. Am J Pathol. 138(3):751-63. PMID: 1705754.

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