Anti-Xyloglucan [LM15] monoclonal antibody
Invented by Prof Paul Knox from PlantProbes
Invented at University of Leeds
- Datasheet
- References (1)
- Inventor Info
Info
| Catalogue Number | 157886 |
| Antigen/Gene or Protein Targets | LM15 xyloglucan epitope, tamarind xyloglucan |
| Synonyms | β(1-4) Xyloglucan |
| Relevance |
Xyloglucan is the major hemicellulose or cross-linking glycan of the primary cell walls of dicotyledons although little is known of its occurrence or functions in relation to cell development and cell wall microstructure. The use of LM15 for the analysis of xyloglucan in the cell walls of tamarind and nasturtium seeds, in which xyloglucan occurs as a storage polysaccharide, indicated that the LM15 xyloglucan epitope occurs throughout the thickened cell walls of the tamarind seed and in the outer regions, adjacent to middle lamellae, of the thickened cell walls of the nasturtium seed. Immunofluorescence analysis of LM15 binding to sections of tobacco and pea stem internodes indicated that the xyloglucan epitope was restricted to a few cell types in these organs. Enzymatic removal of pectic homogalacturonan from equivalent sections resulted in the abundant detection of distinct patterns of the LM15 xyloglucan epitope across these organs and a diversity of occurrences in relation to the cell wall microstructure of a range of cell types. |
| Host | Rat |
| Immunogen | XXXG heptasaccharide of tamarind seed xyloglucan coupled to Bovine serum albumin (BSA). |
| Positive Control | IgG2c |
| Myeloma Used | IR983F |
| Notes |
A neoglycoprotein (XXXG-BSA) was prepared by coupling a heptasaccharide containing 3 xylosyl and 4 glucosyl residues to BSA by reductive amination. Two male Wistar rats were injected with 100 μg XXXG-BSA in complete Freund's adjuvant administered subcutaneously on day 0, with the same amount administered with incomplete Freund's adjuvant on days 33 and 71. On day 145, a selected rat was given a prefusion boost of 100 μg XXXG-BSA in 1 ml PBS by intraperitoneal injection. The spleen was isolated three days later for isolation of lymphocytes and fusion with rat myeloma cell line IR983F. |
| Research Area | Plant Science |
