Anti-SIGLEC1 [HSn 7D2]
Invented by Prof Paul Crocker from University of Dundee
Invented at University of Dundee
- Datasheet
- References (10)
- Inventor Info
Info
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| Catalogue Number | 151199 |
| Applications | ELISA FACS IHC RIA WB |
| Antigen/Gene or Protein Targets | Sialoadhesin domains 1-4 (SIGLEC1) |
| Synonyms | CD169, Sialoadhesin 1 |
| Reactivity | Human |
| Relevance | The siglecs are a family of membrane bound lectins (of the immunoglobulin superfamily) that bind sialic acid and mediate cell-cell interactions. Family members include sialoadhesin, CD22 and CD33. Sialoadhesin is a macrophage-specific cell adhesion molecule that is expressed on activated macrophages in chronic inflammation and in tumours. |
| Host | Mouse |
| Immunogen | Fc fusion protein containing N-terminal 4 domains of human sialoadhesin. |
| Positive Control | Human spleen (IHC), human peripheral blood monocytes (FACS) or MCF7 cells (IF) |
| Subclass | IgG1 |
| Myeloma Used | Sp2/0-Ag14 |
| Recommended Growing Conditions | DMEM + 5% FCS |
| Strain | Balb/c |
| Notes | HSn 7D2 is recommended for cryostat sections and will stain paraffin sections after protease treatment. Detailed procedures for de-paraffinisation are contained in the Steiniger paper. |
| Research Area | Adhesion, Cancer, Immunology |
References: 10 entries
Puryear et al. 2013. PLoS Pathog. 9(4):e1003291. PMID: 23593001.
Interferon-inducible mechanism of dendritic cell-mediated HIV-1 dissemination is dependent on Siglec-1/CD169.
Europe PMC ID: 23593001
Rempel et al. 2008. PLoS One. 3(4):e1967. PMID: 18414664.
Sialoadhesin expressed on IFN-induced monocytes binds HIV-1 and enhances infectivity.
Europe PMC ID: 18414664
Pack et al. 2008. Immunology. 123(3):438-46. PMID: 17944899.
DEC-205/CD205+ dendritic cells are abundant in the white pulp of the human spleen, including the border region between the red and white pulp.
Europe PMC ID: 17944899
Hartnell et al. 2001. Blood. 97(1):288-96. PMID: 11133773.
Characterization of human sialoadhesin, a sialic acid binding receptor expressed by resident and inflammatory macrophage populations.
Europe PMC ID: 11133773
Steiniger et al. 1997. Immunology. 92(2):307-16. PMID: 9415041.
The species-specific structure of microanatomical compartments in the human spleen: strongly sialoadhesin-positive macrophages occur in the perifollicular zone, but not in the marginal zone.
Europe PMC ID: 9415041
Add a reference
References: 10 entries
Puryear et al. 2013. PLoS Pathog. 9(4):e1003291. PMID: 23593001.
Interferon-inducible mechanism of dendritic cell-mediated HIV-1 dissemination is dependent on Siglec-1/CD169.
Rempel et al. 2008. PLoS One. 3(4):e1967. PMID: 18414664.
Sialoadhesin expressed on IFN-induced monocytes binds HIV-1 and enhances infectivity.
Pack et al. 2008. Immunology. 123(3):438-46. PMID: 17944899.
DEC-205/CD205+ dendritic cells are abundant in the white pulp of the human spleen, including the border region between the red and white pulp.
Hartnell et al. 2001. Blood. 97(1):288-96. PMID: 11133773.
Characterization of human sialoadhesin, a sialic acid binding receptor expressed by resident and inflammatory macrophage populations.
Steiniger et al. 1997. Immunology. 92(2):307-16. PMID: 9415041.
The species-specific structure of microanatomical compartments in the human spleen: strongly sialoadhesin-positive macrophages occur in the perifollicular zone, but not in the marginal zone.
Add a reference
