Anti-SIGLEC1 [HSn 7D2]

Tool Details

*FOR RESEARCH USE ONLY

• Name: Anti-SIGLEC1 [HSn 7D2]
• Alternate name: CD169, Sialoadhesin 1
• Clone: HSn 7D2
• Tool sub type: Primary antibody
• Class: Monoclonal
• Conjugation: Unconjugated
• Strain: Balb/c
• Reactivity: Human
• Host: Mouse
• Application: ELISA ; FACS ; IHC ; RIA ; WB
• Description: The siglecs are a family of membrane bound lectins (of the immunoglobulin superfamily) that bind sialic acid and mediate cell-cell interactions. Family members include sialoadhesin, CD22 and CD33. Sialoadhesin is a macrophage-specific cell adhesion molecule that is expressed on activated macrophages in chronic inflammation and in tumours.
• Immunogen: Fc fusion protein containing N-terminal 4 domains of human sialoadhesin.
• Isotype: IgG1
• Myeloma used: Sp2/0-Ag14
• Recommended controls: Human spleen (IHC), human peripheral blood monocytes (FACS) or MCF7 cells (IF)

Target Details

• Target: Sialoadhesin domains 1-4 (SIGLEC1)
• Tissue cell line specificity: Human spleen (IHC), human peripheral blood monocytes (FACS) or MCF7 cells (IF)
• Target background: The siglecs are a family of membrane bound lectins (of the immunoglobulin superfamily) that bind sialic acid and mediate cell-cell interactions. Family members include sialoadhesin, CD22 and CD33. Sialoadhesin is a macrophage-specific cell adhesion molecule that is expressed on activated macrophages in chronic inflammation and in tumours.

Applications

• Application: ELISA ; FACS ; IHC ; RIA ; WB

Handling

• Format: Liquid
• Concentration: 1 mg/ml
• Unit size: 100 ug
• Storage buffer: PBS with 0.02% azide
• Storage conditions: -15° C to -25° C
• Shipping conditions: Shipping at 4° C

References

• Puryear et al. 2013. PLoS Pathog. 9(4):e1003291. PMID: 23593001.
• Interferon-inducible mechanism of dendritic cell-mediated HIV-1 dissemination is dependent on Siglec-1/CD169.
• Rempel et al. 2008. PLoS One. 3(4):e1967. PMID: 18414664.
• Sialoadhesin expressed on IFN-induced monocytes binds HIV-1 and enhances infectivity.
• Pack et al. 2008. Immunology. 123(3):438-46. PMID: 17944899.
• DEC-205/CD205+ dendritic cells are abundant in the white pulp of the human spleen, including the border region between the red and white pulp.
• Hartnell et al. 2001. Blood. 97(1):288-96. PMID: 11133773.
• Characterization of human sialoadhesin, a sialic acid binding receptor expressed by resident and inflammatory macrophage populations.
• Steiniger et al. 1997. Immunology. 92(2):307-16. PMID: 9415041.
• The species-specific structure of microanatomical compartments in the human spleen: strongly sialoadhesin-positive macrophages occur in the perifollicular zone, but not in the marginal zone.