Anti-Rad51 [Rad51 3C10] recombinant antibody
Invented at Absolute Antibody Cancer Research UK London Research Institute: Clare Hall Laboratories
Anti-Rad51 [Rad51 3C10]
Invented by Dr Stephen West from Francis Crick Institute
Invented at Cancer Research UK London Research Institute: Clare Hall Laboratories
- Datasheet
- References (9)
- Inventor Info
Info
| Catalogue Number | 151196 |
| Applications | IHC IP WB |
| Antigen/Gene or Protein Targets | Rad51 |
| Reactivity | Bovine, Human and Pig |
| Relevance |
Monoclonal antibody which binds RAD51 recombinase protein, associated with DNA repair pathway. Background and Research Application RAD51 is a eukaryotic homologue of E. coli RecA, a recombinase, and a component of the homologous recombination DNA repair pathway. RAD51 forms a nucleoprotein filament (through binding RAD52 and single stranded DNA that are exposed following double strand breaks) that initiates recombination. RAD51 catalyses the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. Mutations in the gene can results in breast cancer, mirror movements 2 and Fanconi anaemia. |
| Host | Mouse |
| Immunogen | Full length recombinant human RAD51 protein |
| Immunogen UniProt ID | Q06609 |
| Subclass | IgG1 |
| Myeloma Used | Sp2/0-Ag14 |
| Recommended Growing Conditions | DMEM + 5% FCS |
| Strain | Balb/c |
| Notes |
Production Details Purified using multi-step affinity chromatography with protein A. Storage Conditions Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles. Points of Interest Anti-RAD51 antibody has been used to immunohistochemically stain biopsy and resected specimens of oesophagus and squamous cell carcinoma of the oesophagus. Concentration 1mg/ml as standard |
| Research Area | DNA Damage and Repair, Epigenetics & Nuclear Signalling |
References: 9 entries
Vohhodina et al. 2017. Nucleic Acids Res. 45(22):12816-12833. PMID: 29112714.
Subramanyam et al. 2016. Proc Natl Acad Sci U S A. 113(41):E6045-E6054. PMID: 27671650.
Marampon et al. 2016. Oncotarget. 7(5):5383-400. PMID: 26689991.
Ivanov et al. 2003. J Cell Sci. 116(Pt 20):4095-106. PMID: 12953071.
Endopolyploid cells produced after severe genotoxic damage have the potential to repair DNA double strand breaks.
Europe PMC ID: 12953071
Masson et al. 1999. EMBO J. 18(22):6552-60. PMID: 10562567.
The meiosis-specific recombinase hDmc1 forms ring structures and interacts with hRad51.
Europe PMC ID: 10562567
Barlow et al. 1997. EMBO J. 16(17):5207-15. PMID: 9311981.
Distribution of the Rad51 recombinase in human and mouse spermatocytes.
Europe PMC ID: 9311981
Add a reference
References: 9 entries
Vohhodina et al. 2017. Nucleic Acids Res. 45(22):12816-12833. PMID: 29112714.
Subramanyam et al. 2016. Proc Natl Acad Sci U S A. 113(41):E6045-E6054. PMID: 27671650.
Marampon et al. 2016. Oncotarget. 7(5):5383-400. PMID: 26689991.
Ivanov et al. 2003. J Cell Sci. 116(Pt 20):4095-106. PMID: 12953071.
Endopolyploid cells produced after severe genotoxic damage have the potential to repair DNA double strand breaks.
Masson et al. 1999. EMBO J. 18(22):6552-60. PMID: 10562567.
The meiosis-specific recombinase hDmc1 forms ring structures and interacts with hRad51.
Barlow et al. 1997. EMBO J. 16(17):5207-15. PMID: 9311981.
Distribution of the Rad51 recombinase in human and mouse spermatocytes.
Add a reference
