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Catalogue Number
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157717 |
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Antigen/Gene or Protein Targets
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Prostate Cancer
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Parental Line
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PM151T
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Host
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Human
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Tissue
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Fibroblast
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Disease Keywords
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Prostate Cancer
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Model
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Tumour line
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Relevance
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This cell line presents an in vitro model system which enables the study of human prostate cell differentiation and malignant transformation. Human prostate cells are typically difficult to convert into continuously growing cultures. This cell line is immortalized without viral oncogenes from prostate stromal cells and may serve as a unique experimental platform to permit several research directions, including the study of cell-cell interactions in an authentic prostate microenvironment, prostate cell differentiation, and most significantly, the complex multistep process leading to prostate cell transformation. PMID:16585177.
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Production Details
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PM151 cells derived from a radical prostatectomy specimen of a 62-year-old patient. Pathologic tumor stage was also pT3a, Gleason score 5 (3 + 2). The specimen had a positive margin, meaning that the tumor extended the prostate capsule. Seminal vesicles were tumor-free. (PMID 16585177).
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Conditional
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No
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Research Area
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Cancer
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Growth/Phenotype Keywords
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Fibtroblast-like
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Recommended Growing Conditions
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Reccomend culture in MCDB-131 medium, supplemented with 15% decalcified horse serum, 10 mM HEPES, pH 7.2, 1% of non-essential amino acids solution, 5 µg/ml of insulin, 10 µg/ml of transferrin, 100nM of estradiol, 100 nM of dexamethasone, 5 ng/ml of sodium selenite, 1 ng/ml of basic FGF and 0.1 ng/ml of EGF. Reccomend growth of cultures in a humidified atmosphere of 5% CO2 in air at 37°C. The medium is reccomended ot be changed after 1 week and then every 2 to 3 days. PMID:16585177.
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Cellosaurus ID
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CVCL_LF77
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