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HaCaT CAGA12-Luc/TK Renilla cell line

Invented by Caroline Hill at The Francis Crick Institute

Info

Catalogue Number 156436
Antigen/Gene or Protein Targets Activin and transforming growth factor β (TGF-β) signalling
Parental Line HaCaT cell line
Host Human
Model Reporter
Relevance The human keratinocyte cell line HaCaT modified for use as a reporter cell line for Activin and TGF-beta signalling. The cell line stably expresses CAGA12-Luc, a Smad3/Smad4-dependent reporter containing 12 copies of the CAGAC sites (AGAC motif underlined) from the PAI-1 promoter. This is a Smad3/Smad4-dependent reporter, although it also binds complexes of Smad2 exon 3 with Smad4. The cell line also contains a Renilla reporter driven by the thymidine kinase (TK) promoter to act as an internal control.
Production Details Made by transfecting CAGA12-Luc with pRetroSuper for puromycin resistance and then TK Renilla with pCMV-Bsd forblasticidin resistance.

Clonal
Conditional Description In the presence of activin and TGF-beta signalling luciferase is expressed.
Research Area Cancer, Cell Signaling & Signal Transduction, Developmental Biology
Recommended Growing Conditions Dulbecco's modified Eagle's medium (DMEM) containing 10% fetal calf serum (FCS)

References

There are 1 reference entries for this reagent.

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References: 1 entry

Levy et al. 2007. Mol Cell Biol. 27(17):6068-83. PMID: 17591695.


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References: 1 entry

Levy et al. 2007. Mol Cell Biol. 27(17):6068-83. PMID: 17591695.


Add a reference

Inventor Information