ELISA
FACS
IF
IP
WB
Antigen/Gene or Protein Targets: CEACAM5,6 & 8, CD66e; CD66c; CD66b
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![Sandwich ELISA: solid phase was coated with 3 μg/ml Anti-CEA (Dako) binding human CEACAM1-CEACAM8. After washing, blocking and coating human CEACAM1 antigen, detecting antibody Anti-CEACAM8 [6/40c] (10 μg/ml) followed by HRP-coupled goat anti-mouse Ig was added. TMB was used for visualizing the binding measured by Tecan-ELISA reader at 450 nm.](https://res.cloudinary.com/ximbio/image/upload/c_thumb,fl_lossy,h_124,q_auto,r_max,w_124/bc103f20-6be2-46c3-aacd-31cc205012cd.jpg)
![Sandwich ELISA: solid phase was coated with 3 μg/ml Anti-CEA (Dako) binding human CEACAM1-CEACAM8. After washing, blocking and coating human CEACAM1 antigen, detecting antibody Anti-CEACAM6 [1H7-4B] (10 μg/ml) followed by HRP-coupled goat anti-mouse Ig was added. TMB was used for visualizing the binding measured by Tecan-ELISA reader at 450 nm.](https://res.cloudinary.com/ximbio/image/upload/c_thumb,fl_lossy,h_124,q_auto,r_max,w_124/dd894089-cc88-43d6-ac08-3a7adf8e8fa6.jpg)
![Sandwich ELISA: Solid phase was coated with 3 μg/ml Anti-CEA (Dako) binding human CEACAM1-CEACAM8. After washing, blocking and coating human CEACAM1 antigen, detecting antibody Anti-CEACAM5/8 [5C8C4] (10 μg/ml) followed by HRP-coupled goat anti-mouse Ig was added. TMB was used for visualizing the binding measured by Tecan-ELISA reader at 450 nm.](https://res.cloudinary.com/ximbio/image/upload/c_thumb,fl_lossy,h_124,q_auto,r_max,w_124/d2822a15-4aa8-4320-bfcd-bea7873b63b9.jpg)
![Sandwich ELISA: solid phase was coated with 3 μg/ml Anti-CEA (Dako) binding human CEACAM1-CEACAM8. After washing, blocking and coating human CEACAM1 antigen, detecting antibody Anti-CEACAM3/5 [308/3-3] (10 μg/ml) followed by HRP-coupled goat anti-mouse Ig was added. TMB was used for visualizing the binding measured by Tecan-ELISA reader at 450 nm.](https://res.cloudinary.com/ximbio/image/upload/c_thumb,fl_lossy,h_124,q_auto,r_max,w_124/b27ab7f9-66b5-4f92-a828-181055a68533.jpg)
