Cancer Research Technology
Log in Register
Menu
Search
Monoclonal Antibodies

PC6 Monoclonal Antibody (Clone 8F5)

Invented at Hudson Institute Of Medical Research
  • Datasheet
  • Inventor Info
Print

Info

Image thumbnail for PC6 Monoclonal Antibody (Clone 8F5)
Add an image
Adapted from Heng S, Dynon K, Li Y, Edgell T, Walton K, Rombauts LJ, Vollenhoven B, Nie G. Development of a high-throughput assay for human proprotein convertase 5/6 for detecting uterine receptivity. Anal Biochem. 2015 Apr 15;475:14-21. doi: 10.1016/j.ab.2014.12.012. Epub 2014 Dec 30. PMID: 25554488.Western blots confirming that all three PC6 mAbs (9H12, 8F5 and 4H11) detect PC6 but not PC members PC7 or furin. Blots were also probed with anti-PC7 and anti-furin antibodies to confirm the integrity of PC7 and furin proteins.
Add an image
Image thumbnail for PC6 Monoclonal Antibody (Clone 8F5)
Catalogue Number 154228
Applications IHC WB
Antigen/Gene or Protein Targets Proprotein convertase 5/6A (PC6)
Synonyms PC6
Reactivity Human
Relevance Potential uterine fluid biomarker for endometrium receptivity.
Determining endometrial receptivity is vital in in vitro fertilization (IVF) treatment because the timing of embryo transfer needs to be synchronized with endometrial receptivity. We have previously demonstrated that proprotein convertase 5/6A (PC6) is highly expressed in the receptive endometrium and that PC6 is critical for receptivity establishment in women. Endometrial PC6 is secreted into the uterine fluid, and levels correlate with receptivity status. Detection of PC6 in uterine fluids, therefore, provides a nonsurgical assessment of endometrial receptivity. These PC6 monoclonal antibodies (mAbs, clones 8F5 and 9H12) and can be used to develop a sandwich enzyme-linked immunosorbent assay (ELISA) for PC6 detection in human uterine fluids. The PC6 mAbs were confirmed to be highly specific to PC6, and the ELISA detected PC6 in human uterine fluids with a significantly higher level during the receptive phase. PC6 ELISA provides an important tool in the development of noninvasive strategies to detect endometrial receptivity in women.
Host Mouse
Immunogen Synthetic peptides of human PC6 (PSGYLLDLGMC 857-867 aa; CPPGHYHADK 674-684 aa; NSAVRSIYKASGC 482-494 aa conjugated to keyhole limpet hemocyanin though a C-terminal Cys
Subclass Notes Sp2/0-Ag14
Molecular Weight (kDa) 90
Recommended Growing Conditions Sp2/0 myeloma cells in the presence of 50% (w/v) polyethylene glycol 1500. Cells resuspendedin Hybridoma Serum Free Medium (Invitrogen), supplemented with 10% (w/v) FCS, HAT medium (Invitrogen) and IL-6 (1:1000 dilution).
Strain Balb/c
Notes Use at 15 ug/ml for IHC for human uterine tissues
Research Area Other

References

There are 2 reference entries for this reagent.

View All References

References: 2 entries

Heng et al. 2015. Anal Biochem. 475:14-21. PMID: 25554488.

View  

Development of a high-throughput assay for human proprotein convertase 5/6 for detecting uterine receptivity.

Europe PMC ID: 25554488

View  

Add a reference

References: 2 entries

Heng et al. 2015. Anal Biochem. 475:14-21. PMID: 25554488.

View  

Development of a high-throughput assay for human proprotein convertase 5/6 for detecting uterine receptivity.

View  

Add a reference

Inventor Information

No inventors are currently linked to this reagent.

Add an inventor

Invented at

Hudson Institute Of Medical Research
Hudson Institute Of Medical Research

16 reagents listed on Ximbio