- References (2)
- Inventor Info
|Antigen/Gene or Protein Targets||Proprotein convertase 5/6A (PC6)|
Potential uterine fluid biomarker for endometrium receptivity.
Determining endometrial receptivity is vital in in vitro fertilization (IVF) treatment because the timing of embryo transfer needs to be synchronized with endometrial receptivity. We have previously demonstrated that proprotein convertase 5/6A (PC6) is highly expressed in the receptive endometrium and that PC6 is critical for receptivity establishment in women. Endometrial PC6 is secreted into the uterine fluid, and levels correlate with receptivity status. Detection of PC6 in uterine fluids, therefore, provides a nonsurgical assessment of endometrial receptivity. These PC6 monoclonal antibodies (mAbs, clones 8F5 and 9H12) and can be used to develop a sandwich enzyme-linked immunosorbent assay (ELISA) for PC6 detection in human uterine fluids. The PC6 mAbs were confirmed to be highly specific to PC6, and the ELISA detected PC6 in human uterine fluids with a significantly higher level during the receptive phase. PC6 ELISA provides an important tool in the development of noninvasive strategies to detect endometrial receptivity in women.
|Immunogen||Synthetic peptides of human PC6 (PSGYLLDLGMC 857-867 aa; CPPGHYHADK 674-684 aa; NSAVRSIYKASGC 482-494 aa conjugated to keyhole limpet hemocyanin though a C-terminal Cys|
|Molecular Weight (kDa)||90|
|Recommended Growing Conditions||Sp2/0 myeloma cells in the presence of 50% (w/v) polyethylene glycol 1500. Cells resuspendedin Hybridoma Serum Free Medium (Invitrogen), supplemented with 10% (w/v) FCS, HAT medium (Invitrogen) and IL-6 (1:1000 dilution).|
|Notes||Use at 15 ug/ml for IHC for human uterine tissues|
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