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Water-soluble monovalent copper ligand-1 (MCL-3)

Invented by Christoph J. Fahrni

Info

Catalogue Number 153938
Antigen/Gene or Protein Targets Copper I; Cu(I)
Synonyms MCL-3
Type Fluorescent Probe
Relevance Copper is an essential trace element that is central to a broad range of biological processes, including cellular respiration, connective tissue formation, pigment synthesis, antioxidant defense, and photosynthesis in plants and bacteria. The measurement of reliable Cu(I) protein binding affinities requires competing reference ligands with similar binding strengths; however, the literature on such reference ligands is not only sparse but often conflicting. To address this deficiency, Dr. Fahrni has created and characterized a series of water-soluble monovalent copper ligands, MCL-1, MCL-2, and MCL-3, that form well-defined, air-stable, and colorless complexes with Cu(I) in aqueous solution. X-ray structural data, electrochemical measurements, and an extensive network of equilibrium titrations showed that all three ligands form discrete Cu(I) complexes with 1:1 stoichiometry and are capable of buffering Cu(I) concentrations between 10−10 and 10−17 M.
In vitro applications determination of Cu(I) binding affinities of proteins and small-molecule ligands
Solubility water
Research Area Drug Discovery & Development, Other

References

There are 2 reference entries for this reagent.

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References: 2 entries

Bagchi et al. 2013. J Am Chem Soc. 135(49):18549-59. PMID: 24298878.

Robust affinity standards for Cu(I) biochemistry.

Europe PMC ID: 24298878


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References: 2 entries

Bagchi et al. 2013. J Am Chem Soc. 135(49):18549-59. PMID: 24298878.

Robust affinity standards for Cu(I) biochemistry.


Add a reference

Inventor Information