Cancer Research Technology
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Catalogue Number 153870
Backbone Size (bp) 7866
Bacterial Resistance Ampicillin
Selectable Markers Puromycin
Vector Type Mammalian expression, CRISPR, shuttle
Antigen/Gene or Protein Targets Gt(ROSA)26Sor; ROSA26
Positive Control Contains GFP for positive transfection/integration control
Relevance CRISPR donor vector targeting ROSA26 locus in mouse cells. Contains left and right ROSA26 homology arms with AsisI/MluI cloning site. Use for genomic integration of any gene of interest into ROSA26 locus.

Use in combination with pROSA-GUIDE containing validated gRNA for CAS9 targeting to ROSA26.


Species: Mus musculus (house mouse)
Tag: Myc, FLAG
Cloning method: Restriction Enzyme
5’ cloning site: AsiSI
3’ cloning site: MluI
5’ sequencing primer: CMV Forward
Research Area Gene Expression
Notes Sold for academic research use.If reagent is required for commercialisation purposes, contact Ximbio at enquiries@ximbio.com.

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