Anti-Spectrin [RG/26]
Invented by Dr Jacqueline Cordell from University of Oxford
Invented at University of Oxford
- Datasheet
- References (4)
- Inventor Info
Info
| Catalogue Number | 151403 |
| Applications | IHC IF IP WB |
| Antigen/Gene or Protein Targets | Spectrin |
| Reactivity | Human |
| Relevance | Spectrin is a cytoskeletal protein that lines the intracellular side of the plasma membrane of many cell types in pentagonal or hexagonal arrangements, forming a scaffolding and playing an important role in maintenance of plasma membrane integrity and cytoskeletal structure. In certain types of brain injury such as diffuse axonal injury, spectrin is irreversibly cleaved by the proteolytic enzyme calpain, destroying the cytosketelon. Spectrin cleavage causes the membrane to form blebs and ultimately to be degraded, usually leading to cell death. |
| Host | Mouse |
| Immunogen | Red cell membranes |
| Subclass | IgG2a |
| Myeloma Used | P3/NS1/1-Ag4.1 |
| Recommended Growing Conditions | RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT |
| Notes | Ultroser G can be used at 1% if the cells are not growing well. |
| Research Area | Cardiovascular, Neurobiology, Protein Degradation |
References: 4 entries
Wagner et al. 2014. Vox Sang. 106(1):45-54. PMID: 23909508.
WB
Impact of constant storage temperatures and multiple warming cycles on the quality of stored red blood cells.
Europe PMC ID: 23909508
Sachanonta et al. 2008. Asian Pac J Allergy Immunol. 26(1):37-45. PMID: 18595528.
IHC IF
Host vascular endothelial growth factor is trophic for Plasmodium falciparum-infected red blood cells.
Europe PMC ID: 18595528
Add a reference
References: 4 entries
Wagner et al. 2014. Vox Sang. 106(1):45-54. PMID: 23909508.
WB
Impact of constant storage temperatures and multiple warming cycles on the quality of stored red blood cells.
Sachanonta et al. 2008. Asian Pac J Allergy Immunol. 26(1):37-45. PMID: 18595528.
IHC IF
Host vascular endothelial growth factor is trophic for Plasmodium falciparum-infected red blood cells.
Add a reference
