Anti-p53 [Pab 246]
Invented by David Lane from Karolinska Institutet
Invented at Cancer Research UK London Research Institute: Clare Hall Laboratories
- Datasheet
- References (12)
- Inventor Info
Info
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| Catalogue Number | 151412 |
| Applications | IHC IP |
| Antigen/Gene or Protein Targets | p53 mouse, wild type only |
| Reactivity | Mouse |
| Relevance |
Monoclonal antibody directed against an epitope of p53 within residue 88-93. Background and Research Application Anti-p53 binds p53 protein, a crucial tumour suppressor protein, implicated in over 50% of cancers. p53 is a stress-regulated transcription factor that regulates cell cycle arrest and was first identified as an SV40 large T antigen-binding protein. p53 is involved in many mechanisms of anti-cancer function, and plays a role in apoptosis, genomic stability, cell cycle regulation and inhibition of angiogenesis. p53 is known as the guardian of the genome as it functions to prevent gene mutation. Mutants of p53 which frequently occur in human cancers, fail to recognise their DNA binding site, leading to the loss of tumour suppressor activity. p53 is typically found in low levels in healthy cells due to its short half-life, however in disease states it is expressed in high amounts due to somatic mutations and believed to contribute to transformation and malignancy. p53 mutations are found in over 50% of cancers with germline mutations, offering a predisposition to inherited cancer susceptibility syndrome. Understanding the functions of mutant p53 can help in the development of new therapeutic approaches that may be useful in a broad range of cancer types. The epitope for Pab246 lies within residues 88-93. |
| Host | Mouse |
| Immunogen | SV40-transformed cell line Balb/c SVA31 E7. |
| Immunogen UniProt ID | P02340 |
| Subclass | IgG1 kappa |
| Molecular Weight (kDa) | 50 |
| Myeloma Used | Sp2/0-Ag14 |
| Recommended Growing Conditions | DMEM + 10% FCS or RPMI 1640 + 5% FCS |
| Strain | Balb/c |
| Notes |
Production Details Purified using multi-step affinity chromatography with protein A. Storage Conditions Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles. Points of Interest The epitope for anti-p53 Pab 246 lies on the stretch of amino acids encoded by base pairs 382-450. Concentration 1mg/ml as standard |
| Research Area | Apoptosis and Programmed Cell Death, Cancer, Cell Cycle, DNA Damage and Repair, Epigenetics & Nuclear Signalling |
References: 12 entries
Pradhan et al. 2019. Nucleic Acids Res. 47(4):1637-1652. PMID: 30649466.
Hwang et al. 2018. Cell Rep. 22(1):299-312. PMID: 29298430.
The LIM-only protein FHL2 mediates ras-induced transformation through cyclin D1 and p53 pathways.
Europe PMC ID: 19018287
Labalette et al. 2008. PLoS One. 3(11):e3761. PMID: 19018287.
Han et al. 2008. Cell Stem Cell. 2(3):241-51. PMID: 18371449.
SIRT1 regulates apoptosis and Nanog expression in mouse embryonic stem cells by controlling p53 subcellular localization.
Europe PMC ID: 18371449
Gannon et al. 1990. EMBO J. 9(5):1595-602. PMID: 1691710.
Activating mutations in p53 produce a common conformational effect. A monoclonal antibody specific for the mutant form.
Europe PMC ID: 1691710
Yewdell et al. 1986. J Virol. 59(2):444-52. PMID: 2426467.
Monoclonal antibody analysis of p53 expression in normal and transformed cells.
Europe PMC ID: 2426467
Wade-Evans et al. 1985. EMBO J. 4(3):699-706. PMID: 2408882.
Precise epitope mapping of the murine transformation-associated protein, p53.
Europe PMC ID: 2408882
Add a reference
References: 12 entries
Pradhan et al. 2019. Nucleic Acids Res. 47(4):1637-1652. PMID: 30649466.
Hwang et al. 2018. Cell Rep. 22(1):299-312. PMID: 29298430.
The LIM-only protein FHL2 mediates ras-induced transformation through cyclin D1 and p53 pathways.
Labalette et al. 2008. PLoS One. 3(11):e3761. PMID: 19018287.
Han et al. 2008. Cell Stem Cell. 2(3):241-51. PMID: 18371449.
SIRT1 regulates apoptosis and Nanog expression in mouse embryonic stem cells by controlling p53 subcellular localization.
Gannon et al. 1990. EMBO J. 9(5):1595-602. PMID: 1691710.
Activating mutations in p53 produce a common conformational effect. A monoclonal antibody specific for the mutant form.
Yewdell et al. 1986. J Virol. 59(2):444-52. PMID: 2426467.
Monoclonal antibody analysis of p53 expression in normal and transformed cells.
Wade-Evans et al. 1985. EMBO J. 4(3):699-706. PMID: 2408882.
Precise epitope mapping of the murine transformation-associated protein, p53.
Add a reference
